|
| Status |
Public on Apr 01, 2014 |
| Title |
Gastric tumour GC0109 |
| Sample type |
RNA |
| |
|
| Source name |
Primary gastric tumour
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Primary gastric tumour
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from primary tumors using Trizol extraction and RNeasy midi spin columns (Qiagen) according to the instructions of the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 to 5 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were washed using Affymetrix fluidics stations and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
CEL files were produced using the Affymetrix ® GeneChip ® Command console ® Software (AGCC).
|
| |
|
| Submission date |
Sep 23, 2013 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Rita Ann Busuttil |
| E-mail(s) |
rita.busuttil@petermac.org
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Research
|
| Lab |
Cancer Genetics and Genomics
|
| Street address |
St Andrews Pl
|
| City |
East Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE51105 |
A signature predicting poor prognosis in gastric and ovarian cancer represents a coordinated macrophage and stromal-response. |
|
