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Sample GSM1240794 Query DataSets for GSM1240794
Status Public on Oct 01, 2014
Title Parental
Sample type RNA
 
Source name Parental
Organism Homo sapiens
Characteristics cell line: MCF7
Treatment protocol Cells were grown for 2 days following the growth protocol outlined above and RNA extracted
Growth protocol MCF7 media (Control media): DMEM (Life Technologies), 1X Glutamax (Gibco 35050) and 10% FBS (Biological Industires) when passaging. See growth conditions in Tarragona et al., Journal of Biological Chemistry, 2012), for more details.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the TRIzol® Plus RNA Purification Kit (Life Technologies) and quantified using a Nanodrop spectrophotometer. Quality was assessed using a Bioanalyzer (Agilent) and only high quality samples (RIN ≥9) were used for hybridization.
Label biotin
Label protocol 25 ng total RNA per sample was processed using isothermal amplification SPIA Biotin System (NuGEN Technologies, Inc.)
 
Hybridization protocol 3.75 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a Fluidics Station 450 (Affymetrix, Santa Clara, CA)
Scan protocol GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA)
Description Affymetrix Human Genome U133 Plus 2.0 Array
Data processing Data were quantile normalized and RMA summarized using Bioconductor.
 
Submission date Sep 27, 2013
Last update date Oct 01, 2014
Contact name Evarist Planet Letschert
E-mail(s) evarist.planet@irbbarcelona.org
Organization name IRB Barcelona
Department Biostatistics
Street address C/ Baldiri Reixac 10
City Barcelona
State/province Spain
ZIP/Postal code 08028
Country Spain
 
Platform ID GPL570
Series (2)
GSE51231 Identification of MAF controlled genes in breast cancer cells
GSE51238 16q22-24 DNA amplification specifically predicts and mediates, through MAF gene, breast cancer bone metastasis.

Data table header descriptions
ID_REF
VALUE log2 RMA expression estimate

Data table
ID_REF VALUE
1007_s_at 11.881
1053_at 10.755
117_at 5.96331
121_at 7.47536
1255_g_at 3.41102
1294_at 5.93034
1316_at 6.45378
1320_at 7.90348
1405_i_at 2.9938
1431_at 4.77198
1438_at 6.19491
1487_at 9.35634
1494_f_at 4.21405
1552256_a_at 9.71516
1552257_a_at 11.1039
1552258_at 4.78948
1552261_at 5.39989
1552263_at 6.34243
1552264_a_at 8.46165
1552266_at 6.38729

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM1240794_MPNov10--837-2010--Puro.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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