|
Status |
Public on Oct 15, 2014 |
Title |
sphere_cont-inj_rep2 |
Sample type |
RNA |
|
|
Source name |
Whole sphere stage embryo
|
Organism |
Danio rerio |
Characteristics |
strain: AB developmental stage: sphere treatment: 1nl water
|
Growth protocol |
Embyos were maintained in E3 embryo medium at 28.5 degrees celcius
|
Extracted molecule |
total RNA |
Extraction protocol |
200 embryos were lysed in 1ml Trizol and total RNA extracted according to manufacturers protocol. RNA was then cleaned using QIAGEN Rneasy columns.
|
Label |
Alexa Fluor 555
|
Label protocol |
Alexa Fluor 555 labelled cDNA was produced from 15µg total RNA using Invitrogen Superscript Plus Direct cDNA labelling system according to manufacturers protocol.
|
|
|
Hybridization protocol |
Labelled cDNA was hybridized to Agilent zebrafish V2 expression microarrays according to manufacturers protocol.
|
Scan protocol |
Microarrays were scanned using a GenePix 4000B scanner with GenePix Pro 6 software.
|
Description |
One colour microarray comparison to determine targets of Nodal signaling by overexpression of ndr1 in zebrafish blastulas
|
Data processing |
Spot intensities were extracted using GenePix Pro 6 software and median spot intensities were normalized using the qspline algorithm in oneChannelGUI.
|
|
|
Submission date |
Oct 30, 2013 |
Last update date |
Oct 15, 2014 |
Contact name |
Andrew Christopher Nelson |
E-mail(s) |
a.nelson.1@warwick.ac.uk
|
Organization name |
University of Warwick
|
Department |
School of Life Sciences
|
Street address |
Gibbet Hill Campus
|
City |
Coventry |
ZIP/Postal code |
CV4 7AL |
Country |
United Kingdom |
|
|
Platform ID |
GPL6457 |
Series (2) |
GSE51890 |
Expression profiling of control vs. ndr1 overexpression zebrafish blastulas |
GSE51894 |
Smad2 and Eomesa |
|