|
| Status |
Public on Oct 15, 2014 |
| Title |
sphere_ndr1-inj_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Whole sphere stage embryo
|
| Organism |
Danio rerio |
| Characteristics |
strain: AB
developmental stage: sphere
treatment: 1nl water containing 50pg ndr1 mRNA
|
| Growth protocol |
Embyos were maintained in E3 embryo medium at 28.5 degrees celcius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
200 embryos were lysed in 1ml Trizol and total RNA extracted according to manufacturers protocol. RNA was then cleaned using QIAGEN Rneasy columns.
|
| Label |
Alexa Fluor 555
|
| Label protocol |
Alexa Fluor 555 labelled cDNA was produced from 15µg total RNA using Invitrogen Superscript Plus Direct cDNA labelling system according to manufacturers protocol.
|
| |
|
| Hybridization protocol |
Labelled cDNA was hybridized to Agilent zebrafish V2 expression microarrays according to manufacturers protocol.
|
| Scan protocol |
Microarrays were scanned using a GenePix 4000B scanner with GenePix Pro 6 software.
|
| Description |
One colour microarray comparison to determine targets of Nodal signaling by overexpression of ndr1 in zebrafish blastulas
|
| Data processing |
Spot intensities were extracted using GenePix Pro 6 software and median spot intensities were normalized using the qspline algorithm in oneChannelGUI.
|
| |
|
| Submission date |
Oct 30, 2013 |
| Last update date |
Oct 15, 2014 |
| Contact name |
Andrew Christopher Nelson |
| E-mail(s) |
a.nelson.1@warwick.ac.uk
|
| Organization name |
University of Warwick
|
| Department |
School of Life Sciences
|
| Street address |
Gibbet Hill Campus
|
| City |
Coventry |
| ZIP/Postal code |
CV4 7AL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6457 |
| Series (2) |
| GSE51890 |
Expression profiling of control vs. ndr1 overexpression zebrafish blastulas |
| GSE51894 |
Smad2 and Eomesa |
|
