|
| Status |
Public on Jan 01, 2016 |
| Title |
Wild type Hmel, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type Hmel
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMEL-BRAFV600E
cell type: immortalized melanocytes
overexpression: GFP
|
| Treatment protocol |
Cells were infected with pLenti6.3-RNF2WT or pLenti6.3-GFP lentiviruses, selected with blasticidine (5mg/ml) till control uninfected cells were dead.
|
| Growth protocol |
Cells were maintained in standard tissue culture conditions (5% CO2 , 37oC).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Kit per manufacturer's instructions. cDNA was prepared using SuperScript III using the manufacturer's instructions. qPCR were performed using SybrGreener (Invitrogen) and Stratagene instrument.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
WT_1
Wild-type
Gene expression from wild type human melanocytes- Hmel cells.
|
| Data processing |
Microarray data was normalized by using RMA normalization with the Bioconductor affy package. Data was analyzed using the LIMMA Bioconductor package.
|
| |
|
| Submission date |
Oct 30, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Kadir Caner Akdemir |
| E-mail(s) |
kcakedemir@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE51928 |
Dual roles of RNF2 in melanoma progression [expression] |
| GSE51930 |
Dual roles of RNF2 in melanoma progression |
|
