|
| Status |
Public on Nov 06, 2013 |
| Title |
∆pfap2-g, 15h post-invasion cDNA |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
∆pfap2-g, 15h post-invasion cDNA
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: ∆pfap2-g
timepoint: 15h post-invasion
|
| Treatment protocol |
None
|
| Growth protocol |
Parasites were double synchronized and used to intiate cultures at ~10% parasitemia, media was changed every 12h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
See Kafsack et al. Malaria J 2012 (PMID: 22681930 ) and Painter et al. Methods Mol Biol 2013 (PMID: 22990780) for detailed methods
|
| Label |
Cy5
|
| Label protocol |
See Kafsack et al. Malaria J 2012 (PMID: 22681930 ) and Painter et al. Methods Mol Biol 2013 (PMID: 22990780) for detailed methods
|
| |
|
| Channel 2 |
| Source name |
Shared cDNA reference pool
|
| Organism |
Plasmodium falciparum |
| Characteristics |
reference: Shared cDNA reference pool
|
| Treatment protocol |
None
|
| Growth protocol |
Parasites were double synchronized and used to intiate cultures at ~10% parasitemia, media was changed every 12h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
See Kafsack et al. Malaria J 2012 (PMID: 22681930 ) and Painter et al. Methods Mol Biol 2013 (PMID: 22990780) for detailed methods
|
| Label |
Cy3
|
| Label protocol |
See Kafsack et al. Malaria J 2012 (PMID: 22681930 ) and Painter et al. Methods Mol Biol 2013 (PMID: 22990780) for detailed methods
|
| |
|
| |
| Hybridization protocol |
See Kafsack et al. Malaria J 2012 (PMID: 22681930 ) and Painter et al. Methods Mol Biol 2013 (PMID: 22990780) for detailed methods
|
| Scan protocol |
See Kafsack et al. Malaria J 2012 (PMID: 22681930 ) and Painter et al. Methods Mol Biol 2013 (PMID: 22990780) for detailed methods
|
| Description |
Shared cDNA reference pool
|
| Data processing |
The data were normalized using the Agilent Feature Extractor protocol 9.5.3.1 for two color gene expression (GE2-v5_95_Feb07_no_spikein).
The specific settings are included in the header for each supplementary file.
|
| |
|
| Submission date |
Nov 04, 2013 |
| Last update date |
Sep 12, 2016 |
| Contact name |
Bjorn FC Kafsack |
| Organization name |
Weill Cornell Medicine
|
| Department |
Microbiology & Immunology
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL17880 |
| Series (1) |
| GSE52030 |
Comparison of Gametocyte producer 3D7-A subclone E5 to the gametotcyte nonprodcer strain F12 and pfap2-g deletion mutant |
|
