|
| Status |
Public on Apr 01, 2017 |
| Title |
WT1 |
| Sample type |
RNA |
| |
|
| Source name |
CHUK wild type
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
age: 5-6 weeks
genotype/variation: CHUK wild type
cell type: thymic epithelial cells
|
| Treatment protocol |
Thymuses were minced in cold RPMI-1640 containing 5% FBS. Thymic stromal debris were digested with 0.25% Trypsin-EDTA, and thymic epithelial cells were isolated and further purified by CD45 depletion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRIzol reagent according to the manufacturer?s instruction.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to manufacturer instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to manufacturer instructions.
|
| Scan protocol |
Scanning was performed according to manufacturer instructions.
|
| Description |
Wild Type CHUK, replicate 1
|
| Data processing |
The data were RMA normalized using the Bioconductor affy package (v.1.39.4).
|
| |
|
| Submission date |
Nov 06, 2013 |
| Last update date |
Apr 01, 2017 |
| Contact name |
Sean Davis |
| E-mail(s) |
sdavis2@mail.nih.gov
|
| Phone |
301-435-2652
|
| Organization name |
National Cancer Institute
|
| Lab |
Genetics Branch
|
| Street address |
37 Convent Drive, Room 6138
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE52137 |
Expression data from thymic epithelial cells of 5-6 weeks old C57BL/6J mice. |
|
