|
| Status |
Public on Jan 15, 2014 |
| Title |
103-AHRR-b2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
embryos_72 hpf_AHRRb morpholino
|
| Organism |
Danio rerio |
| Characteristics |
strain: TL
genotype/variation: wild type
developmental stage: 72 hpf embryo
injected with: AHRRb morpholino oligonucleotides
|
| Treatment protocol |
Morpholino oligonucleotides(Control, AHRRa or AHRRb) were injected into 1-2 cell stage zebrafish embryos and sampled at 72 hpf for microarray analysis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using STAT60 protocol
|
| Label |
Cy5
|
| Label protocol |
Labeling was done using Agilent Low Input Quick Amp Labeling kit
|
| |
|
| Channel 2 |
| Source name |
universal reference RNA
|
| Organism |
Danio rerio |
| Characteristics |
strain: TL
genotype/variation: wild type
sample type: universal reference RNA
|
| Treatment protocol |
Morpholino oligonucleotides(Control, AHRRa or AHRRb) were injected into 1-2 cell stage zebrafish embryos and sampled at 72 hpf for microarray analysis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using STAT60 protocol
|
| Label |
Cy3
|
| Label protocol |
Labeling was done using Agilent Low Input Quick Amp Labeling kit
|
| |
|
| |
| Hybridization protocol |
Equal amount of Cy3 (Universal Reference RNA) and Cy5 (individual samples) labeled cRNA probes were mixed and hybridized
|
| Scan protocol |
Genepix 4100A scanner
|
| Description |
Sample 6
|
| Data processing |
Feature Extraction software and data processing using Limma, bioconductor package
|
| |
|
| Submission date |
Nov 08, 2013 |
| Last update date |
Jan 15, 2014 |
| Contact name |
Neelakanteswar Aluru |
| E-mail(s) |
naluru@whoi.edu
|
| Phone |
508-289-3607
|
| Organization name |
Woods Hole Oceanographic Institution
|
| Department |
Biology
|
| Lab |
Aluru Lab
|
| Street address |
45 Water Street
|
| City |
Woods Hole |
| State/province |
MA |
| ZIP/Postal code |
02543 |
| Country |
USA |
| |
|
| Platform ID |
GPL7302 |
| Series (1) |
| GSE52229 |
Knockdown of a zebrafish aryl hydrocarbon receptor repressor (ahrra) affects expression of genes related to photoreceptor development and hematopoiesis. |
|
