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Status |
Public on Jan 08, 2014 |
Title |
Caulobacter_crescentus_DccrM_rep3 |
Sample type |
RNA |
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Source name |
Caulobacter crescentus, delta_CCNA_00382 (ccrM)
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Organism |
Caulobacter vibrioides NA1000 |
Characteristics |
genotype: delta-CCNA_00382 (ccrM)
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Growth protocol |
Cells were cultivated at 28°C in minimal medium (M2G) and harvested in exponential phase (O.D.660nm 0.3).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol and the PureLink RNA kit (Invitrogen); a Dnase I treatment was performed before a second purification on PureLink columns. RNA quality and concentration was determined by analysis with a Bioanalyzer and a Nanodrop.
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Label |
Cy3
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Label protocol |
Labeling was performed following the standard operating protocol of Nimblegen. See www.nimblegen.com.
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Hybridization protocol |
Hybridization was performed following the standard operating protocol of Nimblegen. See www.nimblegen.com.
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Scan protocol |
Scanning was performed on an Agilent High-Resolution Microarray scanner at 2 µm resolution.
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Description |
Caulobacter crescentus, delta_CCNA_00382 (ccrM), replicate 3
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Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package (Roche NimbleGen, Inc.).
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Submission date |
Nov 14, 2013 |
Last update date |
Jan 09, 2014 |
Contact name |
Diego Gonzalez |
Organization name |
University of Lausanne
|
Department |
Department of Fundamental Microbiology
|
Lab |
Prof. Justine Collier
|
Street address |
Quartier UNIL-Sorge, Bâtiment Biophore
|
City |
Lausanne |
ZIP/Postal code |
1015 |
Country |
Switzerland |
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Platform ID |
GPL17931 |
Series (1) |
GSE52375 |
Expression analysis of Caulobacter crescentus NA1000 delta-CCNA_00382 (ccrM) [DccrM] vs Caulobacter crescentus NA1000 wild type [WT] |
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