|
| Status |
Public on Feb 01, 2007 |
| Title |
CASP7_6 antibody |
| Sample type |
protein |
| |
|
| Source name |
specific primary antibody. Antibody description: Caspase-7/MCH-3, Batch number: 26, AbIDNumber: 78
|
| Organism |
Homo sapiens |
| Characteristics |
Each of 176 specific primary antibodies was prescreened to give highest quality by western analysis.
|
| Extracted molecule |
protein |
| Label |
Streptavidin-biotin complex
|
| |
|
| Hybridization protocol |
Each slide was washed manually with deionized water to remove urea. Then, in an Autostainer universal staining system (DAKO), it was blocked with I-block. Also in autostainer, it was incubated with strpetavidin-biotin complex, biotynyl tyramide(for amplification) for 15 min, streptavidin-peroxidase for 15 min, 3,3'-diaminobenzidine-tetrahydrochloride chromagen for 5 min. For detection of total protein, arrays were stained with SYPRO ruby protein blot stain.
|
| Scan protocol |
ProteinChips were scanned with Perfection 1200S scanner (Epson America) with a 256-shade gray scale at 600 dots per inch. Total protein stained with SYPRO ruby was scanned with Fluoriamger SI (Amersham Pharmacia Biotech) at 100-um resolution.
|
| Description |
Each array was incubated with a specific primary antibody. Enables comparison of protein-of-interest across all 60 NCI-60 cell lines. Experimental slide number: GBL00069
|
| Data processing |
The data were analyzed with dose interpolation (DI) algorithm.
|
| |
|
| Submission date |
Aug 11, 2006 |
| Last update date |
Jan 05, 2007 |
| Contact name |
Uma T Shankavaram |
| E-mail(s) |
uma@mail.nih.gov
|
| Phone |
301-496-6718
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
Radiation Oncology Branch
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL4081 |
| Series (1) |
| GSE5501 |
Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types. |
|
