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Status
Public on Feb 01, 2007
Title
NCI-60 cell line Reverse Phase Protein Lysate Array_modified
Technology type
spotted peptide or protein
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
Custom array
Manufacture protocol
Arrays were prepared on nitrocellulose-coated galss slides (FAST Slides, Schleicher & Schuell) by using a pin-in-ring format GMS 417 arrayer (Affymetrix, Santa Clara,CA) with four 500 um diameter pins. Because the cell lysates were viscous, we used pin-in-ring format to avoid clogging of quills. Ten two-fold serial dilutions were made from each lysate. Four 384-well microtiter plates (Genetix, New Milton, Hamphisire, UK) were used to array 640 spots (plus eight spatial registration marks for use in image processing) on a 21x 35 mm area of nitocellulose membrane. The first dilution (four-fold) was made with buffer containing 5M urea, 2% Pharmalyte, pH 8-10.5, and 65 mM DTT. Hence, only the lysate concentration changed along each dilution series. The urea concentration was kept at 6M and CHAPS concentration 2%, to keep proteins in their denatured form. We could remove lysates repeatedly from -80 degreeC storage for use without heating. To avoid evaporation in the microtiter plate during spotting, we kept the humidity in the array chamer at 70-90% with a Vicks ultrasonic humidifier. Arraying was completeed for each microtiter plate within 70 min. Arrays were produced in batches of 20, and the occassional low-quality array (e.g. with many spots dropouts) was discarded.
Support
Glass
Coating
Nitrocellulose
Description
640 spots (plus eight spatial registration marks for use in image processing) on a 21 x 35 mm area of nitocellulose membrane.
Web link
http://www.pnas.org/cgi/doi/10.1073/pnas.2331323100 Contributor(s)
Nishizuka S Citation(s)
14623978 Submission date
Aug 11, 2006
Last update date
Feb 01, 2007
Contact name
Uma T Shankavaram
E-mail(s)
uma@mail.nih.gov Phone
301-496-6718
Organization name
NIH
Department
NCI
Lab
Radiation Oncology Branch
Street address
9000 Rockville Pike
City
Bethesda
State/province
MD
ZIP/Postal code
20892
Country
USA
Samples (176)
GSM126992 , GSM127115 , GSM127116 , GSM127117 , GSM127118 , GSM127119
GSM127120 ,
GSM127121 ,
GSM127122 ,
GSM127123 ,
GSM127124 ,
GSM127125 ,
GSM127126 ,
GSM127127 ,
GSM127128 ,
GSM127129 ,
GSM127130 ,
GSM127131 ,
GSM127132 ,
GSM127133 ,
GSM127134 ,
GSM127135 ,
GSM127136 ,
GSM127137 ,
GSM127138 ,
GSM127139 ,
GSM127140 ,
GSM127141 ,
GSM127142 ,
GSM127143 ,
GSM127144 ,
GSM127145 ,
GSM127146 ,
GSM127147 ,
GSM127148 ,
GSM127149 ,
GSM127150 ,
GSM127151 ,
GSM127152 ,
GSM127153 ,
GSM127154 ,
GSM127155 ,
GSM127156 ,
GSM127157 ,
GSM127158 ,
GSM127159 ,
GSM127160 ,
GSM127161 ,
GSM127162 ,
GSM127163 ,
GSM127164 ,
GSM127165 ,
GSM127166 ,
GSM127167 ,
GSM127168 ,
GSM127169 ,
GSM127170 ,
GSM127171 ,
GSM127172 ,
GSM127173 ,
GSM127174 ,
GSM127175 ,
GSM127176 ,
GSM127177 ,
GSM127178 ,
GSM127179 ,
GSM127180 ,
GSM127181 ,
GSM127182 ,
GSM127183 ,
GSM127184 ,
GSM127185 ,
GSM127186 ,
GSM127187 ,
GSM127188 ,
GSM127189 ,
GSM127190 ,
GSM127191 ,
GSM127192 ,
GSM127193 ,
GSM127194 ,
GSM127195 ,
GSM127196 ,
GSM127197 ,
GSM127198 ,
GSM127199 ,
GSM127200 ,
GSM127201 ,
GSM127202 ,
GSM127203 ,
GSM127204 ,
GSM127205 ,
GSM127206 ,
GSM127207 ,
GSM127208 ,
GSM127209 ,
GSM127210 ,
GSM127211 ,
GSM127212 ,
GSM127213 ,
GSM127214 ,
GSM127215 ,
GSM127216 ,
GSM127217 ,
GSM127218 ,
GSM127219 ,
GSM127220 ,
GSM127221 ,
GSM127222 ,
GSM127223 ,
GSM127224 ,
GSM127225 ,
GSM127226 ,
GSM127227 ,
GSM127228 ,
GSM127229 ,
GSM127230 ,
GSM127231 ,
GSM127232 ,
GSM127233 ,
GSM127234 ,
GSM127235 ,
GSM127236 ,
GSM127237 ,
GSM127238 ,
GSM127239 ,
GSM127240 ,
GSM127241 ,
GSM127242 ,
GSM127243 ,
GSM127244 ,
GSM127245 ,
GSM127246 ,
GSM127247 ,
GSM127248 ,
GSM127249 ,
GSM127250 ,
GSM127251 ,
GSM127252 ,
GSM127253 ,
GSM127254 ,
GSM127255 ,
GSM127256 ,
GSM127257 ,
GSM127258 ,
GSM127259 ,
GSM127260 ,
GSM127261 ,
GSM127262 ,
GSM127263 ,
GSM127264 ,
GSM127265 ,
GSM127266 ,
GSM127267 ,
GSM127268 ,
GSM127269 ,
GSM127270 ,
GSM127271 ,
GSM127272 ,
GSM127273 ,
GSM127274 ,
GSM127275 ,
GSM127276 ,
GSM127277 ,
GSM127278 ,
GSM127279 ,
GSM127280 ,
GSM127281 ,
GSM127282 ,
GSM127283 ,
GSM127284 ,
GSM127285 ,
GSM127286 ,
GSM127287 ,
GSM127288 ,
GSM127289 Series (1)
GSE5501 Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types.
Data table header descriptions ID NCI-60 cell lysate
nci60id panelname age sex priortreat celltype histology source p53 doublingtime institute contributor Reference SPOT_ID spot identifier of NCI-60 cancer cell lysate printed on the array
Data table ID nci60id panelname age sex priortreat celltype histology source p53 doublingtime institute contributor Reference SPOT_ID BT-549 (BR)
NCI60.4
Breast
72
F
epi
Papillary infiltrating ductal carcinoma-mammary gland; breast
Metastasis
53.9
-
W. Coutinho; E.Y. Lasfargues
no pub
NCI-60 cell line: BT-549 (BR)
HS 578T(BR)
NCI60.3
Breast
74
F
epi
Carcinosarcoma-mammary gland; breast
Primary
MT
53.8
Naval Bioscience Laboratory
A.J. Hackett
JNCI 58: 1795-1806,1977
NCI-60 cell line: HS 578T(BR)
MCF-7 (BR)
NCI60.1
Breast
69
F
epi
Adenocarcinoma- mammary gland; breast; metastatic site: pleural effusion;
Pleural effusion
WT
25.4
Michigan Cancer Foundtion
C.M. McGrath
JNCI 51: 1409-1417,1973
NCI-60 cell line: MCF-7 (BR)
NCI ADR-RES(OV)
NCI60.50
Ovarian
F
epi
Adenocarinoma
MT
34
NCI
K.Cowan, NCI
Cancer Res., 46:4087-4090,1986
NCI-60 cell line: NCI ADR-RES(OV)
MDA-MB-231/ATCC (BR)
NCI60.2
Breast
51
F
epi
Adenocarcinoma-mammary gland; breast; epithelial; metastatic site: pleural effusion;
Pleural effusion
MT
41.9
MD Anderson Hospital and Tumor Institute
R. Cailleau
JNCI 53(3): 661-674,1974
NCI-60 cell line: MDA-MB-231/ATCC (BR)
MDA-MB435 (ME)
NCI60.33
Melanoma*
31
F
non-epi
Ductal carcinoma- mammary gland; breast; duct; metastatic site: pleural effusion;
Pleural effusion
MT
25.8
-
R. Cailleau
Cancer Res 40:3118-3129,1980
NCI-60 cell line: MDA-MB435 (ME)
MDA-N (ME)
NCI60.34
Melanoma*
31
F
non-epi
Pleural effusion
MT
22.5
-
-
-
NCI-60 cell line: MDA-N (ME)
T-47D (BR)
NCI60.5
Breast
54
F
epi
infiltrating ductal carcinoma
MT
45.5
-
I. Keydar
Eur J Cancer 15:659-679,1979
NCI-60 cell line: T-47D (BR)
SF-268 (CNS)
NCI60.6
CNS
24
F
Rad
non-epi
Glioblastoma, ud
MT
33.1
-
P.L.Kornblith
Acta Neuropathol 75: 92-103, 1987
NCI-60 cell line: SF-268 (CNS)
SF-295 (CNS)
NCI60.7
CNS
67
F
Rad
non-epi
Glioblastoma, ud
MT
29.5
University of California
M.L. Rosenblum
Acta Neuropathol 75: 92-103, 1988
NCI-60 cell line: SF-295 (CNS)
SF-539 (CNS)
NCI60.8
CNS
34
F
Rad/BCNU/5FU/HU/6MP
non-epi
Glial cell neoplasm
WT
35.4
University of California
M.L. Rosenblum
J Neuropathol Exp Neurol 40: 201-229, 1981
NCI-60 cell line: SF-539 (CNS)
SNB-19 (CNS)
NCI60.9
CNS
47
M
None
non-epi
Glioblastoma, ud
MT
34.6
NINCDS
P.L. Kornblith
Cancer 47: 255, 1981
NCI-60 cell line: SNB-19 (CNS)
SNB-75 (CNS)
NCI60.10
CNS
F
None
non-epi
Astrocytoma
MT
62.8
NINCDS
P.L. Kornblith
-
NCI-60 cell line: SNB-75 (CNS)
U251 (CNS)
NCI60.11
CNS
75
M
non-epi
Glioblastoma,ud
MT
23.8
University of Uppsala, Sweden
-
J Neuropathol Exp Neurol 40: 410-427, 1981
NCI-60 cell line: U251 (CNS)
COLO 205 (CO)
NCI60.12
Colon
70
M
epi
Adenocarcinoma
Ascites
MT
23.8
enver Medical Hospital
G.E. Moore
Can Res. 38: 1345-1355, 1978
NCI-60 cell line: COLO 205 (CO)
HCC-2998 (CO)
NCI60.13
Colon
epi
carcinoma
MT
31.5
MD Anderson Hospital and Tumor Institute
-
-
NCI-60 cell line: HCC-2998 (CO)
HCT-116 (CO)
NCI60.14
Colon
M
epi
carcinoma-vpd
17.4
Baylor College
-
Can Res 41: 1761-1756, 1981
NCI-60 cell line: HCT-116 (CO)
HCT-15 (CO)
NCI60.15
Colon
None
epi
Adenocarcinoma p/md
20.6
ATCC
ATCC (D.L. Dexter, Roger Williams General Hospital)
Can Res 39: 1020-1025, 1979
NCI-60 cell line: HCT-15 (CO)
HT29 (CO)
NCI60.16
Colon
44
F
None
epi
Adenocarcinoma-md
Primary
MT
19.5
Memorial Sloan Kettering Cancer Center
J. Fogh
Human Tumor Cells in vitro: 115-159, 1975
NCI-60 cell line: HT29 (CO)
KM12 (CO)
NCI60.17
Colon
None
epi
Adenocarcinome-pd
MT
23.7
MD Anderson Hospital and Tumor Institute
I.J. Fidler, Univ of Texas M.D. Anderson Hospital and Tumor Institute
Can Res 48: 1943-1948, 1988
NCI-60 cell line: KM12 (CO)
60 10 Kbytes .Supplementary data files not provided
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