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Platform GPL4081 Query DataSets for GPL4081
Status Public on Feb 01, 2007
Title NCI-60 cell line Reverse Phase Protein Lysate Array_modified
Technology type spotted peptide or protein
Distribution non-commercial
Organism Homo sapiens
Manufacturer Custom array
Manufacture protocol Arrays were prepared on nitrocellulose-coated galss slides (FAST Slides, Schleicher & Schuell) by using a pin-in-ring format GMS 417 arrayer (Affymetrix, Santa Clara,CA) with four 500 um diameter pins. Because the cell lysates were viscous, we used pin-in-ring format to avoid clogging of quills. Ten two-fold serial dilutions were made from each lysate. Four 384-well microtiter plates (Genetix, New Milton, Hamphisire, UK) were used to array 640 spots (plus eight spatial registration marks for use in image processing) on a 21x 35 mm area of nitocellulose membrane. The first dilution (four-fold) was made with buffer containing 5M urea, 2% Pharmalyte, pH 8-10.5, and 65 mM DTT. Hence, only the lysate concentration changed along each dilution series. The urea concentration was kept at 6M and CHAPS concentration 2%, to keep proteins in their denatured form. We could remove lysates repeatedly from -80 degreeC storage for use without heating. To avoid evaporation in the microtiter plate during spotting, we kept the humidity in the array chamer at 70-90% with a Vicks ultrasonic humidifier. Arraying was completeed for each microtiter plate within 70 min. Arrays were produced in batches of 20, and the occassional low-quality array (e.g. with many spots dropouts) was discarded.
Support Glass
Coating Nitrocellulose
 
Description 640 spots (plus eight spatial registration marks for use in image processing) on a 21 x 35 mm area of nitocellulose membrane.
 
Web link http://www.pnas.org/cgi/doi/10.1073/pnas.2331323100
Contributor(s) Nishizuka S
Citation(s) 14623978
Submission date Aug 11, 2006
Last update date Feb 01, 2007
Contact name Uma T Shankavaram
E-mail(s) uma@mail.nih.gov
Phone 301-496-6718
Organization name NIH
Department NCI
Lab Radiation Oncology Branch
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Samples (176) GSM126992, GSM127115, GSM127116, GSM127117, GSM127118, GSM127119 
Series (1)
GSE5501 Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types.

Data table header descriptions
ID NCI-60 cell lysate
nci60id
panelname
age
sex
priortreat
celltype
histology
source
p53
doublingtime
institute
contributor
Reference
SPOT_ID spot identifier of NCI-60 cancer cell lysate printed on the array

Data table
ID nci60id panelname age sex priortreat celltype histology source p53 doublingtime institute contributor Reference SPOT_ID
BT-549 (BR) NCI60.4 Breast 72 F epi Papillary infiltrating ductal carcinoma-mammary gland; breast Metastasis 53.9 - W. Coutinho; E.Y. Lasfargues no pub NCI-60 cell line: BT-549 (BR)
HS 578T(BR) NCI60.3 Breast 74 F epi Carcinosarcoma-mammary gland; breast Primary MT 53.8 Naval Bioscience Laboratory A.J. Hackett JNCI 58: 1795-1806,1977 NCI-60 cell line: HS 578T(BR)
MCF-7 (BR) NCI60.1 Breast 69 F epi Adenocarcinoma- mammary gland; breast; metastatic site: pleural effusion; Pleural effusion WT 25.4 Michigan Cancer Foundtion C.M. McGrath JNCI 51: 1409-1417,1973 NCI-60 cell line: MCF-7 (BR)
NCI ADR-RES(OV) NCI60.50 Ovarian F epi Adenocarinoma MT 34 NCI K.Cowan, NCI Cancer Res., 46:4087-4090,1986 NCI-60 cell line: NCI ADR-RES(OV)
MDA-MB-231/ATCC (BR) NCI60.2 Breast 51 F epi Adenocarcinoma-mammary gland; breast; epithelial; metastatic site: pleural effusion; Pleural effusion MT 41.9 MD Anderson Hospital and Tumor Institute R. Cailleau JNCI 53(3): 661-674,1974 NCI-60 cell line: MDA-MB-231/ATCC (BR)
MDA-MB435 (ME) NCI60.33 Melanoma* 31 F non-epi Ductal carcinoma- mammary gland; breast; duct; metastatic site: pleural effusion; Pleural effusion MT 25.8 - R. Cailleau Cancer Res 40:3118-3129,1980 NCI-60 cell line: MDA-MB435 (ME)
MDA-N (ME) NCI60.34 Melanoma* 31 F non-epi Pleural effusion MT 22.5 - - - NCI-60 cell line: MDA-N (ME)
T-47D (BR) NCI60.5 Breast 54 F epi infiltrating ductal carcinoma MT 45.5 - I. Keydar Eur J Cancer 15:659-679,1979 NCI-60 cell line: T-47D (BR)
SF-268 (CNS) NCI60.6 CNS 24 F Rad non-epi Glioblastoma, ud MT 33.1 - P.L.Kornblith Acta Neuropathol 75: 92-103, 1987 NCI-60 cell line: SF-268 (CNS)
SF-295 (CNS) NCI60.7 CNS 67 F Rad non-epi Glioblastoma, ud MT 29.5 University of California M.L. Rosenblum Acta Neuropathol 75: 92-103, 1988 NCI-60 cell line: SF-295 (CNS)
SF-539 (CNS) NCI60.8 CNS 34 F Rad/BCNU/5FU/HU/6MP non-epi Glial cell neoplasm WT 35.4 University of California M.L. Rosenblum J Neuropathol Exp Neurol 40: 201-229, 1981 NCI-60 cell line: SF-539 (CNS)
SNB-19 (CNS) NCI60.9 CNS 47 M None non-epi Glioblastoma, ud MT 34.6 NINCDS P.L. Kornblith Cancer 47: 255, 1981 NCI-60 cell line: SNB-19 (CNS)
SNB-75 (CNS) NCI60.10 CNS F None non-epi Astrocytoma MT 62.8 NINCDS P.L. Kornblith - NCI-60 cell line: SNB-75 (CNS)
U251 (CNS) NCI60.11 CNS 75 M non-epi Glioblastoma,ud MT 23.8 University of Uppsala, Sweden - J Neuropathol Exp Neurol 40: 410-427, 1981 NCI-60 cell line: U251 (CNS)
COLO 205 (CO) NCI60.12 Colon 70 M epi Adenocarcinoma Ascites MT 23.8 enver Medical Hospital G.E. Moore Can Res. 38: 1345-1355, 1978 NCI-60 cell line: COLO 205 (CO)
HCC-2998 (CO) NCI60.13 Colon epi carcinoma MT 31.5 MD Anderson Hospital and Tumor Institute - - NCI-60 cell line: HCC-2998 (CO)
HCT-116 (CO) NCI60.14 Colon M epi carcinoma-vpd 17.4 Baylor College - Can Res 41: 1761-1756, 1981 NCI-60 cell line: HCT-116 (CO)
HCT-15 (CO) NCI60.15 Colon None epi Adenocarcinoma p/md 20.6 ATCC ATCC (D.L. Dexter, Roger Williams General Hospital) Can Res 39: 1020-1025, 1979 NCI-60 cell line: HCT-15 (CO)
HT29 (CO) NCI60.16 Colon 44 F None epi Adenocarcinoma-md Primary MT 19.5 Memorial Sloan Kettering Cancer Center J. Fogh Human Tumor Cells in vitro: 115-159, 1975 NCI-60 cell line: HT29 (CO)
KM12 (CO) NCI60.17 Colon None epi Adenocarcinome-pd MT 23.7 MD Anderson Hospital and Tumor Institute I.J. Fidler, Univ of Texas M.D. Anderson Hospital and Tumor Institute Can Res 48: 1943-1948, 1988 NCI-60 cell line: KM12 (CO)

Total number of rows: 60

Table truncated, full table size 10 Kbytes.




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