|
Status |
Public on Feb 01, 2007 |
Title |
MCC antibody |
Sample type |
protein |
|
|
Source name |
specific primary antibody. Antibody description: MCC, Batch number: 26, AbIDNumber: 127
|
Organism |
Homo sapiens |
Characteristics |
Each of 176 specific primary antibodies was prescreened to give highest quality by western analysis.
|
Extracted molecule |
protein |
Label |
Streptavidin-biotin complex
|
|
|
Hybridization protocol |
Each slide was washed manually with deionized water to remove urea. Then, in an Autostainer universal staining system (DAKO), it was blocked with I-block. Also in autostainer, it was incubated with strpetavidin-biotin complex, biotynyl tyramide(for amplification) for 15 min, streptavidin-peroxidase for 15 min, 3,3'-diaminobenzidine-tetrahydrochloride chromagen for 5 min. For detection of total protein, arrays were stained with SYPRO ruby protein blot stain.
|
Scan protocol |
ProteinChips were scanned with Perfection 1200S scanner (Epson America) with a 256-shade gray scale at 600 dots per inch. Total protein stained with SYPRO ruby was scanned with Fluoriamger SI (Amersham Pharmacia Biotech) at 100-um resolution.
|
Description |
Each array was incubated with a specific primary antibody. Enables comparison of protein-of-interest across all 60 NCI-60 cell lines. Experimental slide number: GBL00062
|
Data processing |
The data were analyzed with dose interpolation (DI) algorithm.
|
|
|
Submission date |
Aug 11, 2006 |
Last update date |
Jan 05, 2007 |
Contact name |
Uma T Shankavaram |
E-mail(s) |
uma@mail.nih.gov
|
Phone |
301-496-6718
|
Organization name |
NIH
|
Department |
NCI
|
Lab |
Radiation Oncology Branch
|
Street address |
9000 Rockville Pike
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL4081 |
Series (1) |
GSE5501 |
Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types. |
|