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Sample GSM127286 Query DataSets for GSM127286
Status Public on Feb 01, 2007
Title VASP antibody
Sample type protein
 
Source name specific primary antibody. Antibody description: VASP, Batch number: 13, AbIDNumber: 203
Organism Homo sapiens
Characteristics Each of 176 specific primary antibodies was prescreened to give highest quality by western analysis.
Extracted molecule protein
Label Streptavidin-biotin complex
 
Hybridization protocol Each slide was washed manually with deionized water to remove urea. Then, in an Autostainer universal staining system (DAKO), it was blocked with I-block. Also in autostainer, it was incubated with strpetavidin-biotin complex, biotynyl tyramide(for amplification) for 15 min, streptavidin-peroxidase for 15 min, 3,3'-diaminobenzidine-tetrahydrochloride chromagen for 5 min. For detection of total protein, arrays were stained with SYPRO ruby protein blot stain.
Scan protocol ProteinChips were scanned with Perfection 1200S scanner (Epson America) with a 256-shade gray scale at 600 dots per inch. Total protein stained with SYPRO ruby was scanned with Fluoriamger SI (Amersham Pharmacia Biotech) at 100-um resolution.
Description Each array was incubated with a specific primary antibody. Enables comparison of protein-of-interest across all 60 NCI-60 cell lines. Experimental slide number: 26573
Data processing The data were analyzed with dose interpolation (DI) algorithm.
 
Submission date Aug 11, 2006
Last update date Jan 05, 2007
Contact name Uma T Shankavaram
E-mail(s) uma@mail.nih.gov
Phone 301-496-6718
Organization name NIH
Department NCI
Lab Radiation Oncology Branch
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4081
Series (1)
GSE5501 Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types.

Data table header descriptions
ID_REF NCI60 cell line lysate on the platform
VALUE Adjusted DI25 values in log2 scale.
SIGNAL_RAW Signal intensity processed by DI25 algorithm. The values represent in log2 scale.
TotalProtein Intensity of total protein used for the adjustment of protein expression.

Data table
ID_REF VALUE SIGNAL_RAW TotalProtein
BT-549 (BR) 0.95017903 3.5134 2.56322097
HS 578T(BR) -0.5806016 1 1.5806016
MCF-7 (BR) 2.19515806 4.8683 2.67314194
NCI ADR-RES(OV) -1.786 1 2.786
MDA-MB-231/ATCC (BR) -2.1997919 1 3.1997919
MDA-MB435 (ME) 2.96369677 6.6644 3.70070323
MDA-N (ME) -2.7886613 1 3.7886613
T-47D (BR) 1.8406629 4.9104 3.0697371
SF-268 (CNS) 1.01224032 3.9625 2.95025968
SF-295 (CNS) -0.3080145 2.2254 2.5334145
SF-539 (CNS) -1.5253435 1 2.5253435
SNB-19 (CNS) -0.9997048 1 1.9997048
SNB-75 (CNS) -1.7736629 1 2.7736629
U251 (CNS) -0.125329 2.2624 2.387729
COLO 205 (CO) 2.26951452 6.8403 4.57078548
HCC-2998 (CO) -1.2296935 1 2.2296935
HCT-116 (CO) 3.29391613 6.5632 3.26928387
HCT-15 (CO) 1.79502419 5.1448 3.34977581
HT29 (CO) 1.70621613 4.6982 2.99198387
KM12 (CO) 1.64202419 5.5213 3.87927581

Total number of rows: 60

Table truncated, full table size 2 Kbytes.




Supplementary data files not provided

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