|
| Status |
Public on Dec 13, 2013 |
| Title |
t-AML patient_390369-A02 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
chip DNA from skin
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: therapy-related Acute Myeloid Leukemia (t-AML) patient
tissue: normal (skin)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Isolation of Genomic DNA from Cryopreserved Cells using the QIAamp DNA Blood Mini Kit
|
| Label |
Cy5
|
| Label protocol |
DNAs labeled according to manufacturer's recommendations (NG_CGH_guide_v5p1) using Nimblegen's Dual-Color DNA Labeling Kit (p/n 05223547001)
|
| |
|
| Channel 2 |
| Source name |
chip DNA from bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: therapy-related AML (t-AML) patient
tissue: tumor (bone marrow)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Isolation of Genomic DNA from Cryopreserved Cells using the QIAamp DNA Blood Mini Kit
|
| Label |
Cy3
|
| Label protocol |
DNAs labeled according to manufacturer's recommendations (NG_CGH_guide_v5p1) using Nimblegen's Dual-Color DNA Labeling Kit (p/n 05223547001)
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing according to manufacturer's recommendations (NG_CGH_guide_v5p1) using Nimblegen's Hybridization Kit (p/n 05223474001) and Washing Buffer kit (p/n 05223504001)
|
| Scan protocol |
Arrays were scanned on an Axon 4000B scanner per Nimblegen's protocol (NG_CGH_guide_v5p1)
|
| Description |
390369_A02
557772
|
| Data processing |
Arrays were processed using NimbleScan v2.5
|
| |
|
| Submission date |
Dec 12, 2013 |
| Last update date |
Dec 13, 2013 |
| Contact name |
Matt Walter |
| E-mail(s) |
mjwalter@dom.wustl.edu
|
| Organization name |
washington university in st.louis school of medicine
|
| Department |
oncology
|
| Street address |
4940 parkview
|
| City |
st.louis |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL18053 |
| Series (2) |
| GSE53250 |
The DNA Double-Strand Break Response Is Abnormal in Myeloblasts From Patients With Therapy-Related Acute Myeloid Leukemia [NimbleGen] |
| GSE53251 |
The DNA Double-Strand Break Response Is Abnormal in Myeloblasts From Patients With Therapy-Related Acute Myeloid Leukemia |
|
