|
| Status |
Public on Mar 31, 2014 |
| Title |
R_lesional_day15 |
| Sample type |
RNA |
| |
|
| Source name |
Lesional skin biopsy from psoriasis patient, 140mg brodalumab treated, day 15
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: psoriasis
tissue: lesional skin
treatment: 140mg brodalumab
time: day 15
patient: R
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The frozen biopsy was homogenized using a TissueRuptor, and RNA was extracted using the mirVana™ kit with on-column DNase treatment.
|
| Label |
biotin
|
| Label protocol |
Total RNA was reverse-transcribed and amplified using the NuGen Ovation™ RNA amplification system V2 with Ovation™WB reagent. Amplified RNA was fragmented and labeled using the NuGen FL-Ovation™ cDNA Biotin Module V2. Amplification and labeling were performed on a Beckman-Coulter ArrayPlex.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix protocol.
|
| Scan protocol |
Procedure used standard Affymetrix protocol.
|
| Description |
skn55787
|
| Data processing |
Scans were imported into Rosetta Resolver and pre-processed using the 'Resolver Affymetrix Rosetta Intensity Profile Builder (saves reporters)' pipeline.
|
| |
|
| Submission date |
Dec 20, 2013 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Jeannette Bigler |
| E-mail(s) |
jeannette.bigler@bjgroupllc.com
|
| Organization name |
BJ Group, LLC
|
| Street address |
3200 NE 92nd Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE53552 |
Gene expression profiling in psoriatic lesional and non-lesional skin [brodalumab treatment] |
|
