|
| Status |
Public on Apr 01, 2020 |
| Title |
Vasectomy_mouse2 |
| Sample type |
RNA |
| |
|
| Source name |
1002_vasectomy
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
protocol: vasectomy
tissue: lateral prostate
|
| Treatment protocol |
Vasectomy or sham surgery (control) were performed at the age of 16 weeks.
|
| Growth protocol |
C57BL/6 mice were performed vasectomy or sham surgery at the age of 16 weeks and harvested at the age of 24 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
biotin labeling using Affymetrix standard WT plus protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
Gene expression from prostate 24 weeks after surgery
Vasectomy2
|
| Data processing |
Normalization by Affymetrix Expression Console Software, version 1.3.1 – RMA (default settings)
|
| |
|
| Submission date |
Jan 11, 2014 |
| Last update date |
Apr 01, 2020 |
| Contact name |
TAKASHI KAWAHARA |
| E-mail(s) |
takashi_tk2001@yahoo.co.jp
|
| Organization name |
The Johns Hopkins Hospital
|
| Department |
Pathology
|
| Lab |
Pathology (Urology)
|
| Street address |
600 N. Wolfe Street, Marburg 148
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE54003 |
Expression data from mouse with vasectomy or sham surgery |
|
