|
Status |
Public on Apr 05, 2014 |
Title |
CC_A_rep2 |
Sample type |
RNA |
|
|
Source name |
good quality embryo_CCs
|
Organism |
Homo sapiens |
Characteristics |
embryo quality: good quality tissue: oocytes at metaphase II stage cell type: Cumulus Cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from each sample was quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis.
|
Label |
Cy3
|
Label protocol |
Agilent Quick Amp Labeling Kit was used for sample labeling.
|
|
|
Hybridization protocol |
Hybridization was performed in Agilent’s SureHyb Hybridization Chambers.
|
Scan protocol |
After washing, slides were scanned with the Agilent DNA Microarray Scanner.
|
Description |
5A
|
Data processing |
Data was extracted using Agilent Feature Extraction software. Results were provided in the Raw Intensity.xls. Normalization was performed using the Agilent GeneSpring GX v12.1 software.
|
|
|
Submission date |
Jan 16, 2014 |
Last update date |
Apr 05, 2014 |
Contact name |
Dawei Chen |
E-mail(s) |
chdawei1987@gmail.com
|
Organization name |
USTC
|
Department |
Life Sciences School
|
Street address |
Huangshan Rd.
|
City |
Hefei |
State/province |
Anhui |
ZIP/Postal code |
230021 |
Country |
China |
|
|
Platform ID |
GPL15314 |
Series (1) |
GSE54135 |
Molecular signature in human cumulus cells related to embryonic developmental potential |
|