|
| Status |
Public on Apr 05, 2014 |
| Title |
CC_B_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
bad quality embryo_CCs
|
| Organism |
Homo sapiens |
| Characteristics |
embryo quality: bad quality
tissue: oocytes at metaphase II stage
cell type: Cumulus Cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each sample was quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Agilent Quick Amp Labeling Kit was used for sample labeling.
|
| |
|
| Hybridization protocol |
Hybridization was performed in Agilent’s SureHyb Hybridization Chambers.
|
| Scan protocol |
After washing, slides were scanned with the Agilent DNA Microarray Scanner.
|
| Description |
5B
|
| Data processing |
Data was extracted using Agilent Feature Extraction software. Results were provided in the Raw Intensity.xls. Normalization was performed using the Agilent GeneSpring GX v12.1 software.
|
| |
|
| Submission date |
Jan 16, 2014 |
| Last update date |
Apr 05, 2014 |
| Contact name |
Dawei Chen |
| E-mail(s) |
chdawei1987@gmail.com
|
| Organization name |
USTC
|
| Department |
Life Sciences School
|
| Street address |
Huangshan Rd.
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230021 |
| Country |
China |
| |
|
| Platform ID |
GPL15314 |
| Series (1) |
| GSE54135 |
Molecular signature in human cumulus cells related to embryonic developmental potential |
|
