|
| Status |
Public on Sep 15, 2014 |
| Title |
Un RAW macrophages 2011-12-15 |
| Sample type |
RNA |
| |
|
| Source name |
Uninfected macrophages
|
| Organism |
Mus musculus |
| Characteristics |
sample type: macrophages
infection: none
|
| Treatment protocol |
Macrophages were infected with opsonized bacteria so as to achieve 80% infectivity of cells as determined by an uptake assay
|
| Growth protocol |
Murine RAW264.7 macrophages were cultured in complete DMEM media at 37 degrees in a CO2 incubator; Mycobacteria was cultured in 7H9 broth with OADC at 37 degrees incubator shaker
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using MirVana kit (Ambion) and treated with DNAse1 (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed using standard labeling procedures from Nimblegen and following manufacture's instructions
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Nimblegen protocols
|
| Scan protocol |
Scanning was performed according to Nimblegen protocol
|
| Description |
Cells
|
| Data processing |
The raw data was subject to RMA using Nimblescan, quantile normalization
|
| |
|
| Submission date |
Jan 27, 2014 |
| Last update date |
Sep 15, 2014 |
| Contact name |
prachi singh |
| Organization name |
university of notredame
|
| Department |
biological sciences
|
| Street address |
130 Galvin
|
| City |
Notredame |
| State/province |
indiana |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
|
| Platform ID |
GPL10572 |
| Series (1) |
| GSE54419 |
Host transcripts detected in exosomes derived from M.tb infected murine RAW264.7 macrophages |
|
