|
| Status |
Public on Jan 06, 2015 |
| Title |
peripheral airway epithelial cells, smoker 2, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral airway epithelial cells_smoker
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: smoker 2
age: 62
gender: male
case: smoker
cell type: peripheral airway epithelial cells
|
| Treatment protocol |
The cytology brush was spun down and the cell pellet was collected and stored at -80° C until RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extracted total RNA using Qiagen miRNeasy Mini Kit (Valencia, CA) according to the company protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Ten µg of cRNA was hybridized to the array, and was processed with the Affymetrix system, including GeneChip Hybridization Oven 640
|
| Scan protocol |
GeneChips scanned with Fluidics Station 450 and Scanner 3000.
|
| Description |
JT2
|
| Data processing |
Normalization of Affymetrix array data was by Robust Multichip Average (RMA) through the RMexpress software package (http://rmaexpress.bmbolstad.com/).
|
| |
|
| Submission date |
Jan 29, 2014 |
| Last update date |
Jan 07, 2015 |
| Contact name |
Jun-Chieh James Tsay |
| E-mail(s) |
tsayj01@nyumc.org
|
| Organization name |
NYU Langone Medical Center
|
| Department |
Medicine
|
| Street address |
562 First Ave. NBV 7N27
|
| City |
new york |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE54495 |
Field of Cancerization in Peripheral Airway Epithelium: Gene Expresssion |
| GSE54543 |
Field of Cancerization in Peripheral Airway Epithelium |
|
