|
| Status |
Public on Apr 30, 2016 |
| Title |
Chicken_54_LB 50 |
| Sample type |
RNA |
| |
|
| Source name |
posterior right site of the cerebrum
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: brain
layer line: Lohmann Brown
age: 65-68 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were extracted without meninges and separately stored in reaction tubes filled with 1.5 ml RNAlater™. Isolation of total RNA was made using the RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Ambion® WT Express Kit User Manual).
|
| |
|
| Hybridization protocol |
A total of 5.5 μg of the fragmented biotinylated cRNAs were hybridized for 16 hours at +45 °C on Affymetrix ChiGene-1_0-st Arrays. Microarrays were washed and stained with Streptavidin Phycoerythrin using the Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
Microarrays were scanned using the Affymetrix GCS3000 Scanner.
|
| Description |
Gene expression data from two different strains of laying hens from a small group housing system
|
| Data processing |
Image analysis was processed with GCOS1.2 Software Suite (Affymetrix). Normalization of the raw microarray data was performed with the Robust Multi array Average method (RMA) and GeneSpring GX 11 Software was used for statistical analysis.
|
| |
|
| Submission date |
Mar 04, 2014 |
| Last update date |
Apr 30, 2016 |
| Contact name |
Christin Habig |
| E-mail(s) |
christin.habig@tiho-hannover.de
|
| Organization name |
Institute for Animal Breeding and Genetics
|
| Street address |
Buenteweg 17p
|
| City |
Hannover |
| State/province |
lower saxony |
| ZIP/Postal code |
30559 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18368 |
| Series (1) |
| GSE55570 |
Gene expression data of two different strains of laying hens from a small group housing system |
|
