|
| Status |
Public on Aug 29, 2017 |
| Title |
LGIN tissue sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
LGD
|
| Organism |
Homo sapiens |
| Characteristics |
age: 61
gender: Male
disease state: gastric low-grade intraepithelial neoplasia
tissue: gastric tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit(Qiagen, MD, USA). The concentration was measured by ND-1000 UV-VIS spectrophotometer(NanoDrop Technologies, DE, USA). The quality of purified RNA(RNA integrity number, RIN) was detected by the RNA 6000 Labchip Kit and Agilent 2100 Bioanalyzer(Agilent, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
Initially 100ng of total RNA was added into the reaction to generate 1.65µg Cy3-labeled cRNA using Low Input Quick Amp Labeling Kit(Agilent).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Agilent 4×44K Whole Human Genome microarray.
|
| Scan protocol |
Slides were washed and then scanned with the Agilent G2505B Microarray Scanner System. Fluorescence intensities of the scanned images were extracted and preprocessed with the Agilent Feature Extraction Software (v9.1).
|
| Description |
gene expression of gastric low-grade intraepithelial neoplasia
|
| Data processing |
Data normalization, Quality Control, Principal component analysis(PCA) and Filter by Flags were performed using the GeneSpring software GX 12.6(Agilent Technologies).
|
| |
|
| Submission date |
Mar 07, 2014 |
| Last update date |
Aug 29, 2017 |
| Contact name |
Xue Xu |
| E-mail(s) |
rabbit316@sina.com
|
| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
ShuaiFuYuan 1
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE55696 |
Gene expression profiling of gastric early-stage carcinoma and precancerous lesions |
|
