|
| Status |
Public on Feb 26, 2015 |
| Title |
Fatbody_UV12h_sample2 |
| Sample type |
RNA |
| |
|
| Source name |
fatbody, 3 day of fifth instar larvae, domestic silkworm, Kinshu x Showa
|
| Organism |
Bombyx mori |
| Characteristics |
tissue: fatbody
developmental stage: 3day fifth instar larvae
stress: UV
time: 12h
|
| Treatment protocol |
Silkworms were reared on fresh mulberry leaves and kept at 25 degrees Celsius on a 12-hour light/12-hour dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from control or UV treated fatbody were isolated using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was quantified on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA)
|
| Label |
Cy3
|
| Label protocol |
Total RNA(300ng) was processed for Cy3-labelled cRNA synthesis using the Agilent Quick Amp Labeling Kit according to the manufacturer's instructions (Agilent Technologies). The Cy3-labelled cRNA was purified using RNeasy Mini Kit (Qiagen) and quantified on an Agilent 2100 and Nano Drop 1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Hybridization was performed using the Gene Expression Hybridization Kit (Agilent Technologies). 1.65ug of Cy3-labelled cRNA was mixed, fragmented and hybridized to the silkworm 4 x 44 K oligo-microarray slide at 65 degrees Celsius for 17 hours with shaking at 10 rpm. The array was washed in Agilent Gene Expression Wash Buffer 1 for 1min at room temperature and Agilent Gene Expression Wash Buffer 2 for 1min at 37 degrees Celsius.
|
| Scan protocol |
Intensities of the hybridized probes were detected with an Agilent G2565B Microarray Scanner at 10-mm scan resolution.
|
| Description |
Gene expression in 3day fifth instar larvae of UV treatment for 12 hours
|
| Data processing |
Signals were extracted with G2565AA Feature Extraction Software v.9.5 (Agilent Techonologies). The raw data in text files were normalized 'per spot' and 'per chip' using GeneSpring GX v.10.3 (Agilent Techonologies).
|
| |
|
| Submission date |
Mar 12, 2014 |
| Last update date |
Feb 26, 2015 |
| Contact name |
Hiromasa Ono |
| E-mail(s) |
hono@dbcls.rois.ac.jp
|
| Organization name |
Research Organization of Information and Systems
|
| Department |
Database Center for Life Science (DBCLS)
|
| Lab |
Mishima Lab.
|
| Street address |
National Institute of Genetics, Yata 1111
|
| City |
Mishima |
| State/province |
Shizuoka |
| ZIP/Postal code |
411-8540 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15525 |
| Series (1) |
| GSE55816 |
Gene expression of ultra violet irradiation of silk worm Bombyx mori larvae |
|
