wt C57Bl/6 mice were intraperitoneally (i.p.) injected with CL316243 (1 mg/kg in 0.1 ml) once daily for 3 consecutive days. Stromal vascular fraction (SVF) from gWAT of vehicle- and CL-treated mice were fractioned by a collagenase digestion method. The SVF were served as RNA extraction.
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
Encore biotin module(4200,Nugen) followed the manufacturer’s instructions to obtain biotin labeled cDNA.
Hybridization protocol
Array hybridization and wash was performed using GeneChip Hybridization, wash and stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00‐0331‐220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00‐0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Scan protocol
Slides were scanned by GeneChip® Scanner 3000 (Cat#00‐00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description
treatment:CL316243, 1 mg/kg, 3 days
Data processing
Raw data were normalized by RMA algorithm, Expression Console.
