|
| Status |
Public on Oct 27, 2014 |
| Title |
cervix_paired non-tumor_2ad |
| Sample type |
RNA |
| |
|
| Source name |
paired adjacent non-tumor tissues
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: cervix
cancer status: non-tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs are harvested using TRIzol (Invitrogen) and the RNeasy kit (Qiagen) according to manufacturer’s instructions, including a DNase digestion step.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix GeneChip WT Terminal Labeling Kit protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix hGlue3_0 transcriptome array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
The arrays were scanned using Affymetrix Fluidics Station FS450 and GeneChip Scanner 30007G.
|
| Description |
Gene expression data from cervical cacner sample without chemoradiotherapy
|
| Data processing |
Affymetrix Power Tools (APT) was applied for data processing. Arrays were RMA-background-corrected, quantile-normalized and summarized into exon/gene expression using median-polish approaches. The probe group file is hGlue3_0.r1.pgf, and the meta-probeset file is hGlue3_0.r1.TC.mps
|
| |
|
| Submission date |
Mar 16, 2014 |
| Last update date |
Oct 27, 2014 |
| Contact name |
chen ye |
| E-mail(s) |
yechen12358@qq.com
|
| Organization name |
the second military medical university
|
| Street address |
Xiangyin road, no.800
|
| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL16238 |
| Series (1) |
| GSE55940 |
Human transcriptome expression analysis of cervical cancer |
|
