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Sample GSM1367118 Query DataSets for GSM1367118
Status Public on Jan 01, 2015
Title Cortical astrocytes 7DIV, Mecp2 WT/y, biological rep 4
Sample type RNA
 
Source name P0 mouse cortical astrocytes 7DIV, Mecp2 WT/y
Organism Mus musculus
Characteristics strain: C57Bl6J
cell type: Primary culture of astrocytes from cortex
genotype: Mecp2 WT/y
age: P0 pups, 7 days in vitro
Growth protocol P0 newborns from pregnant Mecp2 308/+ heterozygous mice were used for the preparation of dissociated cortical astrocyte cultures. Astrocytes were kept 7 days in vitro in DMEM supplemented with 10% FBS and 1% Pen/Strep (GIBCO)
Extracted molecule total RNA
Extraction protocol Total RNA extraction with the Qiagen RNeasy kit (Qiagen) as described by the manufacturer
Label Biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
 
Hybridization protocol Following fragmentation, cDNA were hybridized for 16 hr at 45C on GeneChip murine Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Description Gene expression data
Data processing The data were preprocessed and analyzing using Partek Genomic Suite.
 
Submission date Apr 10, 2014
Last update date Jan 01, 2015
Contact name Florent Dumont
E-mail(s) florent.dumont@u-psud.fr
Organization name Université Paris-Saclay
Street address 17 avenue des sciences
City 91400 Orsay
State/province Ile de France
ZIP/Postal code 91400
Country France
 
Platform ID GPL16570
Series (1)
GSE56709 Astrocyte transcriptome from Mecp2-deficient mice

Data table header descriptions
ID_REF
VALUE RMA normalized signal

Data table
ID_REF VALUE
17200001 4.438146
17200003 4.53911
17200005 2.159525
17200007 3.072948
17200009 4.029002
17200011 3.912565
17200013 4.402438
17200015 5.443851
17200017 4.083251
17200019 3.253773
17200021 3.192856
17200023 6.355171
17200025 5.042613
17200027 5.918165
17200029 2.296106
17200031 2.497882
17200033 2.7758
17200035 2.344027
17200037 4.650345
17200039 3.189446

Total number of rows: 41345

Table truncated, full table size 722 Kbytes.




Supplementary file Size Download File type/resource
GSM1367118_ech12_MoGene-2_0-st.CEL.gz 8.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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