|
| Status |
Public on Aug 17, 2014 |
| Title |
B16-F1 tumor established in Stat2 KO, Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Stat2 KO_B16 melanoma tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6
genotype/variation: Stat2 KO
inoculated with: B16-F1 melanoma tumor cells
tissue: B16 melanoma tumor establised in mice
|
| Treatment protocol |
Individual tumors established in WT or S2KO mice were harvested at day 20 of the study and snapped frozen in liquid nitrogen prior to RNA extraction with TRIzol.
|
| Growth protocol |
Tumor cells growing in the log-phase at 37C were collected by trypsinization, washed with PBS twice and resuspended at 5x106/mL prior to the inoculation of mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) and further purified by RNeasy kit (Qiagen, Valencia, CA). The quality of total RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
RNA samples were labeled and hybridized to the Affymetrix mouse Gene 2.0 ST Array according to the manufacturer's instructions
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
S2KO2
|
| Data processing |
The data were analyzed using the Affymetrix Gene Expression Console Software, version 1.3.1 with RMA normalization algorithm.
|
| |
|
| Submission date |
Apr 15, 2014 |
| Last update date |
Aug 17, 2014 |
| Contact name |
Ana Maria Gamero |
| E-mail(s) |
gameroa@temple.edu
|
| Phone |
215-707-1268
|
| Organization name |
Temple University
|
| Department |
Biochemistry
|
| Street address |
3440 N. Broad St.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE56792 |
Gene expression analysis of B16-F1 melanoma tumors established in WT and Stat2KO mice |
|
