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Sample GSM1370132 Query DataSets for GSM1370132
Status Public on Apr 16, 2014
Title sictrl with glucose, biological rep2
Sample type RNA
 
Source name A549 Cells
Organism Homo sapiens
Characteristics treatment: sictrl with glucose
cell line: A549
Treatment protocol Cells were transfected with control or SRC-1 targeting siRNA using DharmaFECT 1 according to the manufacturer's protocol. Transfected cells were changed to medium supplemented with 10% dialyzed FCS and incubated for 36 hours before the indicated treatment with DMEM with or without glucose supplemented with 10% dialyzed FCS.
Growth protocol A549 cell lines were cultured in DMEM supplemented with 10% fetal calf serum (FCS), penicillin, and streptomycin (100 U/mL). Twenty-four hours prior to transfection, A549 cells were plated in 6-well plates.
Extracted molecule total RNA
Extraction protocol RNAs was isolated using the RNeasy mini Kit (QIAGEN).
Label biotin
Label protocol Biotinylated cRNA was produced using the standard Affymetrix protocol.
 
Hybridization protocol 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip Genome Array. GeneChips were washed, then stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned with the GeneChip Scanner 3000.
Description siControl with glucose
Data processing The data were analyzed using RMAExpress Version: 1.0.4 with default analysis settings.
 
Submission date Apr 16, 2014
Last update date Apr 28, 2014
Contact name David Lonard
E-mail(s) dlonard@bcm.edu
Organization name Baylor College of Medicine
Department Mol & Cell Biology-Mol.Regulation
Lab BCM-Michael Debakey Center, Room M809, MS: BCM130
Street address 1 Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL570
Series (1)
GSE56843 Steroid Receptor Coactivator 1 is an Integrator of Glucose and NAD(+)/NADH Homeostasis

Data table header descriptions
ID_REF
VALUE RMA quantile normalized data

Data table
ID_REF VALUE
1007_s_at 10.294388
1053_at 9.205479
117_at 5.175406
121_at 7.339156
1255_g_at 2.649701
1294_at 4.699476
1316_at 6.023787
1320_at 4.975629
1405_i_at 2.860975
1431_at 3.693926
1438_at 5.169263
1487_at 7.875716
1494_f_at 5.576419
1552256_a_at 9.895942
1552257_a_at 8.310725
1552258_at 4.601823
1552261_at 4.05958
1552263_at 6.290112
1552264_a_at 7.162503
1552266_at 2.735178

Total number of rows: 54675

Table truncated, full table size 1055 Kbytes.




Supplementary file Size Download File type/resource
GSM1370132_irs_BO_9177_H2_35927.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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