|
| Status |
Public on Apr 16, 2014 |
| Title |
siSRC1 with glucose, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 Cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: siSRC1 with glucose
cell line: A549
|
| Treatment protocol |
Cells were transfected with control or SRC-1 targeting siRNA using DharmaFECT 1 according to the manufacturer's protocol. Transfected cells were changed to medium supplemented with 10% dialyzed FCS and incubated for 36 hours before the indicated treatment with DMEM with or without glucose supplemented with 10% dialyzed FCS.
|
| Growth protocol |
A549 cell lines were cultured in DMEM supplemented with 10% fetal calf serum (FCS), penicillin, and streptomycin (100 U/mL). Twenty-four hours prior to transfection, A549 cells were plated in 6-well plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs was isolated using the RNeasy mini Kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was produced using the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip Genome Array. GeneChips were washed, then stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned with the GeneChip Scanner 3000.
|
| Description |
siSRC-1 with glucose
|
| Data processing |
The data were analyzed using RMAExpress Version: 1.0.4 with default analysis settings.
|
| |
|
| Submission date |
Apr 16, 2014 |
| Last update date |
Apr 28, 2014 |
| Contact name |
David Lonard |
| E-mail(s) |
dlonard@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Mol & Cell Biology-Mol.Regulation
|
| Lab |
BCM-Michael Debakey Center, Room M809, MS: BCM130
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56843 |
Steroid Receptor Coactivator 1 is an Integrator of Glucose and NAD(+)/NADH Homeostasis |
|
