|
| Status |
Public on Jan 07, 2015 |
| Title |
SUP-B8 GSK126 0.5 uM at 72 hrs, Biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
SUP-B8 GSK126 0.5 uM at 72 hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SUP-B8
treatment: 0.5 uM GSK126
time: 72 hrs
|
| Treatment protocol |
Cell lines were treated for 72 hours with DMSO or 0.5 uM EZH2 inhibitor GSK126
|
| Growth protocol |
Cancer cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from SUP-B8 GSK126 0.5 uM at 72 hrs
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and robust multi-array average (RMA )as normalization method. Log2 Transformed
|
| |
|
| Submission date |
Apr 21, 2014 |
| Last update date |
Jan 07, 2015 |
| Contact name |
Matteo Righi |
| E-mail(s) |
m.righi@autolus.com
|
| Organization name |
Autolus Therapeutics
|
| Street address |
The MediaWorks, 191 Wood Ln
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
W12 7FP |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56954 |
A687V EZH2 is a driver of histone H3 lysine 27 (H3K27) hyper-trimethylation |
|
