|
| Status |
Public on Apr 24, 2014 |
| Title |
ARDS lung Biological Replicate 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control lung tissues
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Whole lung
strain: Sprague Dawley
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Tri reagent following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
3DNA 50 labeling kit
|
| |
|
| Channel 2 |
| Source name |
ARDS lung tissues
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
tissue: Whole lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Tri reagent following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
3DNA 50 labeling kit
|
| |
|
| |
| Hybridization protocol |
The two-step microarray hybridization was carried out with the 3DNA 50 Expression kit (Genisphere Inc., Hatfield, PA)
|
| Scan protocol |
The hybridized slides were scanned with ScanArray Express
|
| Description |
Replicate 4 of 7. ARDS-CON.
F5 and F3 correspond to Cy5 and Cy3 channels, respectively
|
| Data processing |
Raw data were extracted from the DNA microarray hybridization images with GenePix Pro 5. Spot image visualization, spot quality evaluation, data normalization, and SAM test for the identification of the differentially expressed genes were performed using the RealSpot software.
|
| |
|
| Submission date |
Apr 23, 2014 |
| Last update date |
Apr 24, 2014 |
| Contact name |
CHAOQUN HUANG |
| E-mail(s) |
chaoqh@okstate.edu
|
| Phone |
405-744-3410
|
| Organization name |
Oklahoma State University
|
| Department |
Physiological Sciences
|
| Lab |
Lundberg-Kienlen Lung Biology and Toxicology Laboratory
|
| Street address |
264 McElroy Hall
|
| City |
Stillwater |
| State/province |
OK |
| ZIP/Postal code |
74078 |
| Country |
USA |
| |
|
| Platform ID |
GPL1699 |
| Series (1) |
| GSE57011 |
Gene expression profiles in rat Acute Respiratory Distress Syndrome (ARDS) |
|
