|
| Status |
Public on Jun 08, 2015 |
| Title |
H1 OCT4:GFP hESC_2 |
| Sample type |
RNA |
| |
|
| Source name |
cell culture
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1 OCT4:GFP
cell type: embryonic stem cell
pluripotency state: primed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell pellets were lysed in TRIzol (Invitrogen), total RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to a custom gene expression microarray containing probes for all protein coding genes and 15902 lncRNA genes (AMADID# 041648).
|
| Scan protocol |
Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
|
| Data processing |
Probe intensities were extracted using Feature Extraction software (Agilent).
|
| |
|
| Submission date |
May 05, 2014 |
| Last update date |
Jun 08, 2015 |
| Contact name |
Pieter mestdagh |
| E-mail(s) |
pieter.mestdagh@ugent.be
|
| Organization name |
Center for Medical Genetics
|
| Street address |
De Pintelaan 185
|
| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL18641 |
| Series (1) |
| GSE57271 |
Novel culture conditions facilitating the induction of naïve pluripotency in human embryonic stem cells |
|
