|
| Status |
Public on Apr 21, 2015 |
| Title |
BM_Ter119+_Polg_mutant_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
BM_Ter119+_Polg_mutant
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
strain: offspring from mating Atg7 flox/flox Epo-cre wt/wt PolG wt/mt (female) X Atg7 flox/flox Epo-cre ki/wt PolG wt/mt (male)
genotype/variation: Polgtm1Lrsn mt/mt
tissue: bone marrow
cell type: Ter119+ cells
|
| Treatment protocol |
None
|
| Growth protocol |
Ter119 positive cells from bone marrow were separated via autoMACS® Pro Sepaerator (Miltenyi Biotec) through depletion of CD45 with CD45 MicroBeads (130-052-301, Miltenyi Biotec) and then Ter119 positive selection by Ter119 microbeads (130-049-901, Miltenyi Biotec).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using RNeasy Mini Kit (Cat. No. 74104, QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Sense target cDNA was prepared from 100ng of total RNA using the Ambion® WT Expression Kit (Life Technologies P/N 4411973). cDNA was fragmented and biotin-labeled using the Affymetrix® GeneChip® WT Terminal Labeling Kit (Affymetrix P/N 900671).
|
| |
|
| Hybridization protocol |
Five micrograms of cDNA was hybridized for 16h at 45C on a Mouse Gene 2.0 ST GeneChip array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Polg_mutant_rep3
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.5
|
| |
|
| Submission date |
May 16, 2014 |
| Last update date |
Apr 23, 2015 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE57761 |
Mito-protective autophagy is impaired in erythroid cells of aged mtDNA-mutator mice |
|
