|
| Status |
Public on Nov 15, 2006 |
| Title |
IR treatment 4h timepoint, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
TK6 cells 4hour IR treat
|
| Organism |
Homo sapiens |
| Characteristics |
normal human diploid lymphoblast immortalized cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion miRVANA RNA isolation kit according to manufacturer's protocol
|
| Label |
Cy5
|
| Label protocol |
The 3’ ends of the RNA molecules were tailed and labeled using the mirVana™ miRNA Labeling Kit (Ambion) according to the manufacturer’s instructions. Amine-modified nucleotides were incorporated during the poly (A) polymerase mediated tailing reaction, and Cy5 succinimide esters (Amersham Biosciences (GE Healthcare)) were conjugated to the amine moieties on the microRNAs
|
| |
|
| Hybridization protocol |
Labelled miRNA were hybridized for 16 hr at 42C on miRVANA Bioarrays. Following hybridization, bioarrays were washed 3x and dried.
|
| Scan protocol |
Bioarrays were scanned using the GenePix 4200AL scanner.
|
| Description |
Gene expression data from cells treated with 2.5 Gy g-IR and collected at 4h.
|
| Data processing |
The fluorescent signal associated with the probes and local background was extracted using GenePix Pro and background adjusted fluorescent values were normalized for each microRNA using a variation stabilization transformation method described by Huber et al
|
| |
|
| Submission date |
Oct 12, 2006 |
| Last update date |
Oct 12, 2006 |
| Contact name |
Carmen J Marsit |
| E-mail(s) |
cmarsit@hsph.harvard.edu
|
| Organization name |
Harvard School of Public Health
|
| Department |
Genetics and Complex Diseases
|
| Lab |
Kelsey
|
| Street address |
665 Huntington Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL4411 |
| Series (1) |
| GSE6020 |
MiRNA Expression data from Tk6 cell treated with arsenic, gamma-IR, or grown under folate-deficiency |
|
