|
| Status |
Public on Oct 19, 2006 |
| Title |
cDNA_A_cy3 |
| Sample type |
RNA |
| |
|
| Source name |
E. coli MG1655 RNA
|
| Organism |
Escherichia coli |
| Characteristics |
Strain MG1655
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified on a RNeasy columns (Qiagen); ribosomal RNAs were removed with the MicroExpress kit (Ambion). The resulting RNA was reverse transcribed with SuperScript II reverse transcriptase (Invitrogen) + random hexamers + dNTP mix containing aminoallyl-dUTP.
|
| Label |
Cy3
|
| Label protocol |
cDNA was labeled with functionalized fluorophore.
|
| |
|
| Hybridization protocol |
Labeled DNA was hybridized and scanned using proprietary methods (NimbleGen).
|
| Scan protocol |
Labeled DNA was hybridized and scanned using proprietary methods (NimbleGen).
|
| Description |
Body-labeled cDNA
|
| Data processing |
Linearly scaled so that intensity values in gDNA_A_cy5, cDNA_A_cy3, gDNA_B_cy3, and cDNA_B_cy5 are comparable
|
| |
|
| Submission date |
Oct 17, 2006 |
| Last update date |
Oct 18, 2006 |
| Contact name |
Nikos Reppas |
| Organization name |
Harvard University
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL4458 |
| Series (2) |
| GSE6060 |
E. coli MG1655 transcriptome inferred from a tiled microarray |
| GSE6069 |
The transition between transcriptional initiation and elongation in E. coli is highly variable and often rate-limiting |
|
