|
| Status |
Public on Oct 02, 2014 |
| Title |
No RU486 treatment replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
heads of actin-GSG>UAS-ATPSyn-dRNAi
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
gender: female
|
| Treatment protocol |
Flies were treated with and without 200 uM RU486 until they reached the age of 2-week
|
| Growth protocol |
Females were cultured on the diet with 2% sugar and 18% yeast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
3.5 ug total RNA was labeled with the 3DNA array kit following manufacturer's instructions (Genisphere, Hatfield, PA).
|
| |
|
| Channel 2 |
| Source name |
Heads of Canton S
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
gender: female
|
| Treatment protocol |
Flies were treated with and without 200 uM RU486 until they reached the age of 2-week
|
| Growth protocol |
Females were cultured on the diet with 2% sugar and 18% yeast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
3.5 ug total RNA was labeled with the 3DNA array kit following manufacturer's instructions (Genisphere, Hatfield, PA).
|
| |
|
| |
| Hybridization protocol |
Samples were added to custom printed long oligo arrays
|
| Scan protocol |
The hybridized slides were scanned with the GenePix 4100A Scanner (Axon Instruments, Sunnyvale, CA) and images were analyzed with GenePix Pro 6 software.
|
| Description |
Biological replicate 2
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal using BRB-ArrayTools (http://linus.nci.nih.gov/BRB-ArrayTools.html, Biological research Branch, NCI)
|
| |
|
| Submission date |
Jun 24, 2014 |
| Last update date |
Oct 02, 2014 |
| Contact name |
Sige Zou |
| E-mail(s) |
zous@mail.nih.gov
|
| Phone |
410-558-8461
|
| Organization name |
National Institute on Aging
|
| Department |
Translational Gerontology Branch
|
| Lab |
Zou lab
|
| Street address |
251 Bayview Blvd
|
| City |
Baltiimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL18860 |
| Series (1) |
| GSE58778 |
Mitochondrial ATP Synthase Subunit d Interacts with TOR Signaling and Dietary Macronutrients to Modulate Proteostasis and Lifespan in Drosophila |
|
