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Sample GSM1420258 Query DataSets for GSM1420258
Status Public on Jun 25, 2015
Title N293
Sample type RNA
 
Source name Tg+ F2 male
Organism Mus musculus
Characteristics gender: male
tissue: mouse prostate tumor
tumor_burden: 0.4
Treatment protocol Prostate tumor sample were collected and snap frozen in liquid nitrogen, then stored at -80 degrees until ready for RNA extraction
Growth protocol Transgene-positive F2 male tumor growth and metastasis were quantified either at 30 weeks or when humane experimental endpoints were reached.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tumor samples using QIAGEN Rneasy mini kit
Label biotin
Label protocol Labeling performed using GeneChip® Terminal Labeling kit according to manufacturer's protocol.
 
Hybridization protocol The hybridization cocktail containing the fragmented and labeled cDNAs were hybridized to The Affymetrix mouse GeneChip® 2.0 ST chips. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix.
Scan protocol An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
Description TRAMPxNOD no metastasis
Data processing Data was processed using Partek
 
Submission date Jun 25, 2014
Last update date Oct 09, 2019
Contact name Kendra Williams
Organization name NIH
Department NHGRI
Lab Nigel Crawford
Street address 50 South Dr, Bldg 50
City Bethesda
State/province MD
ZIP/Postal code 20814
Country USA
 
Platform ID GPL16570
Series (1)
GSE58829 Expression data from prostate tumor of TRAMPxNOD/ShiLtJ F2 cross

Data table header descriptions
ID_REF
VALUE Normalized log2 expression intensity

Data table
ID_REF VALUE
17200001 7.37848
17200003 7.68424
17200005 6.69587
17200007 5.08543
17200009 7.11415
17200011 6.84685
17200013 6.62
17200015 6.81322
17200017 3.16328
17200019 5.31369
17200021 5.31424
17200023 7.57512
17200025 6.89674
17200027 7.77955
17200029 7.27776
17200031 5.35374
17200033 4.67293
17200035 4.55423
17200037 6.56543
17200039 5.09682

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.




Supplementary file Size Download File type/resource
GSM1420258_N293_MoGene-2_0-st_.CEL.gz 9.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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