|
| Status |
Public on Mar 25, 2015 |
| Title |
wt_spb464_1 |
| Sample type |
RNA |
| |
|
| Source name |
wt_spb464_1
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
strain: spb464
genotype/variation: ade6-hp+
|
| Growth protocol |
Strains grown at 30 degress in YES medium to OD600=0.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the hot phenol method
|
| Label |
biotin
|
| Label protocol |
200ng total RNA was processed according to the GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual from Affymetrix (GeneChip Whole Transcription Sense Target Labeling technical manual, Rev. 2).
|
| |
|
| Hybridization protocol |
hybridization was performed for 16h at 45°C and 60rpm in an Affymetrix hybridization oven; washing steps were done in an Affymetrix Fluidics station with the Affymetrix protocol FS450_0002
|
| Scan protocol |
GeneChips were scanned with Affymetrix GCC Scan Control v. 3.0.0.1214 on a GeneChip® Scanner 3000 with autoloader.
|
| Data processing |
oligo-level data was rma background corrected, quantile normalized and log2 transformed using Bioconductor packages tilingArray and preprocessCore on R and the probe annotation in the file Sp20b_M_v04_probes_to_sPombe.tab
RMA normalized log2 expression values are available in the file Normalized_data.txt on the series level.
|
| |
|
| Submission date |
Jul 07, 2014 |
| Last update date |
Mar 25, 2015 |
| Contact name |
Tim Roloff |
| E-mail(s) |
tim.roloff@fmi.ch
|
| Organization name |
FMI
|
| Department |
Functional Genomics
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7715 |
| Series (2) |
| GSE59169 |
RNA expression profiling for Paf1 complex mutant strains [mRNA] |
| GSE59171 |
RNA expression profiling for Paf1 complex mutant strains |
|
