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Status |
Public on Mar 25, 2015 |
Title |
Paf1D_spb1953_2 |
Sample type |
RNA |
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Source name |
Paf1D_spb1953_2
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
strain: spb1953 genotype/variation: Paf1Δ, ade6-hp+
|
Growth protocol |
Strains grown at 30 degress in YES medium to OD600=0.5
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the hot phenol method
|
Label |
biotin
|
Label protocol |
200ng total RNA was processed according to the GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual from Affymetrix (GeneChip Whole Transcription Sense Target Labeling technical manual, Rev. 2).
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Hybridization protocol |
hybridization was performed for 16h at 45°C and 60rpm in an Affymetrix hybridization oven; washing steps were done in an Affymetrix Fluidics station with the Affymetrix protocol FS450_0002
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Scan protocol |
GeneChips were scanned with Affymetrix GCC Scan Control v. 3.0.0.1214 on a GeneChip® Scanner 3000 with autoloader.
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Data processing |
oligo-level data was rma background corrected, quantile normalized and log2 transformed using Bioconductor packages tilingArray and preprocessCore on R and the probe annotation in the file Sp20b_M_v04_probes_to_sPombe.tab RMA normalized log2 expression values are available in the file Normalized_data.txt on the series level.
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Submission date |
Jul 07, 2014 |
Last update date |
Mar 25, 2015 |
Contact name |
Tim Roloff |
E-mail(s) |
tim.roloff@fmi.ch
|
Organization name |
FMI
|
Department |
Functional Genomics
|
Street address |
Maulbeerstrasse 66
|
City |
Basel |
ZIP/Postal code |
4058 |
Country |
Switzerland |
|
|
Platform ID |
GPL7715 |
Series (2) |
GSE59169 |
RNA expression profiling for Paf1 complex mutant strains [mRNA] |
GSE59171 |
RNA expression profiling for Paf1 complex mutant strains |
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