|
| Status |
Public on Jul 11, 2014 |
| Title |
LRH-1 WT jejunum, biological repliate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse jejunum WT5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: jejunum
genotype: LRH-1 WT
|
| Treatment protocol |
Mice were fed ad libitum before beeing sacrificed.
|
| Growth protocol |
Mice are housed 5 per cage in IVC GM500 cages (Tecniplast, 500 cm2) according to the Swiss cantonal rules.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was amplified using Ambion® WT Expression Kit from life technologies (Part Number 4411974) according to manufactures protocols, then 5500ng cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit (part number 900671) according to manufactures protocols.
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized according to Affymetrix protocols. ~16hr incubation and then washed and stained according to Affymetrix protocols on Affymetrix 450 Fluidics Station.
|
| Scan protocol |
Arrays were scanned on Affymetrix GSC3000 7G Scanner
|
| Description |
Gene expression data
|
| Data processing |
Samples were analyzed using Affymetrix Expression Console, Graphpad Prism, and Gene Set Enrichment Analysis (Broad Institute).
|
| |
|
| Submission date |
Jul 10, 2014 |
| Last update date |
Jul 11, 2014 |
| Contact name |
Kristina Schoonjans |
| E-mail(s) |
admin.auwerx@epfl.ch
|
| Organization name |
Ecole Polytechnique Fédérale de Lausanne
|
| Department |
SV IBI GR-SCH
|
| Lab |
Metabolic signaling
|
| Street address |
Station 15
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE59303 |
Global hepatic transcript data from LRH-1 WT and LRH-1 K289R jejunums |
| GSE59333 |
Global transcript analysis of livers and/or jejunums of liver-specific Lrh-1 knockout as well as Lrh-1 K289R knockin mice |
|
