|
| Status |
Public on Jul 16, 2014 |
| Title |
Control PBMCs Replicate 14 |
| Sample type |
RNA |
| |
|
| Source name |
Control PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood mononuclear cells (PBMCs)
age: 67
Sex: Male
Stage: Healthy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-labe
|
| |
|
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
The microarray slides were scanned using Gene-Pix 4000B (Axon Instruments Inc., Foster City, California, USA), which used a 532nm laser for Cy3 measurement and a 635nm laser for Cy5 measurement.
|
| Description |
Biological replicate 14 of 100. Control peripheral blood mononuclear cells, untreated, harvested after several passages.
|
| Data processing |
Sixteen-bit TIFF images produced by the Axon scanner were analyzed using the GenePixPro 3.0 software package (Axon Instruments Inc.). Data were normalized using the Global normalization method.
|
| |
|
| Submission date |
Jul 15, 2014 |
| Last update date |
Jul 16, 2014 |
| Contact name |
Xi Wang |
| E-mail(s) |
231115210@qq.com
|
| Organization name |
Xi‘an Jiaotong University Health Science Center
|
| Street address |
No.76 Yanta West Road
|
| City |
Xi'an |
| ZIP/Postal code |
710061 |
| Country |
China |
| |
|
| Platform ID |
GPL18887 |
| Series (1) |
| GSE59446 |
Human Peripheral blood mononuclear cells (PBMCs): Control vs. Kashin-Beck Disease |
|
