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Sample GSM1438947 Query DataSets for GSM1438947
Status Public on Feb 23, 2015
Title Tietar_leaves at T1 fully irrigated
Sample type RNA
Source name Tietar T1 control
Organism Zea mays
Characteristics variety: Tietar
developmental stage: vegetative eight-leaves (V8) (T1 stage)
growth condition: fully irrigated
tissue: leaves
Treatment protocol Leaves were collected from plants at the vegetative eight-leaves (V8) (T1 stage) and at the silking (R1) (T2 stage), all at the same time of the day, immediately frozen in liquid nitrogen and stored at -80°C
Growth protocol Seeds were sown at a density of 80,000 plants/ha and were treated following standard agricultural practices. Each plot was fully irrigated until plants reached the vegetative six-leaf (V6) stage; from V6 to harvest they were irrigated differently : plot 1 was irrigated at 100% field capacity (control); plot 2 was not irrigated (0% field capacity, stress 2).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Spectrum® Plant Total RNA Kit (Sigma) according to the manufacturer's instruction
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Maize Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChip 3000 scanner device
Description CONT1T
Data processing Partek Genomics Suite versione 6.5
Submission date Jul 17, 2014
Last update date Feb 24, 2015
Contact name Mariolina Gulli
Organization name University of Parma
Department Life Sciences
Street address Parco Area delle Scienze 11a
City Parma
ZIP/Postal code 43124
Country Italy
Platform ID GPL4032
Series (1)
GSE59533 Expression data from Zea mays cultivars Tietar and DKC 6575

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 334.6115
AFFX-BioB-M_at 459.3845
AFFX-BioB-3_at 480.6306
AFFX-BioC-5_at 1054.337
AFFX-BioC-3_at 1378.657
AFFX-BioDn-5_at 2383.477
AFFX-BioDn-3_at 5783.161
AFFX-CreX-5_at 16946.35
AFFX-CreX-3_at 20086.65
AFFX-DapX-5_at 135.3939
AFFX-DapX-M_at 697.6689
AFFX-DapX-3_at 1167.758
AFFX-LysX-5_at 113.4717
AFFX-LysX-M_at 114.7499
AFFX-LysX-3_at 334.7092
AFFX-PheX-5_at 84.54777
AFFX-PheX-M_at 94.69724
AFFX-PheX-3_at 137.7005
AFFX-ThrX-5_at 9.170675
AFFX-ThrX-M_at 75.09213

Total number of rows: 17734

Table truncated, full table size 448 Kbytes.

Supplementary file Size Download File type/resource
GSM1438947_R736_3_CONT1T_Maize_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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