|
| Status |
Public on Feb 23, 2015 |
| Title |
Tietar_leaves at T1 fully irrigated |
| Sample type |
RNA |
| |
|
| Source name |
Tietar T1 control
|
| Organism |
Zea mays |
| Characteristics |
variety: Tietar
developmental stage: vegetative eight-leaves (V8) (T1 stage)
growth condition: fully irrigated
tissue: leaves
|
| Treatment protocol |
Leaves were collected from plants at the vegetative eight-leaves (V8) (T1 stage) and at the silking (R1) (T2 stage), all at the same time of the day, immediately frozen in liquid nitrogen and stored at -80°C
|
| Growth protocol |
Seeds were sown at a density of 80,000 plants/ha and were treated following standard agricultural practices. Each plot was fully irrigated until plants reached the vegetative six-leaf (V6) stage; from V6 to harvest they were irrigated differently : plot 1 was irrigated at 100% field capacity (control); plot 2 was not irrigated (0% field capacity, stress 2).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Spectrum® Plant Total RNA Kit (Sigma) according to the manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Maize Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChip 3000 scanner device
|
| Description |
CONT1T
|
| Data processing |
Partek Genomics Suite versione 6.5
|
| |
|
| Submission date |
Jul 17, 2014 |
| Last update date |
Feb 24, 2015 |
| Contact name |
Mariolina Gulli |
| Organization name |
University of Parma
|
| Department |
Life Sciences
|
| Street address |
Parco Area delle Scienze 11a
|
| City |
Parma |
| ZIP/Postal code |
43124 |
| Country |
Italy |
| |
|
| Platform ID |
GPL4032 |
| Series (1) |
| GSE59533 |
Expression data from Zea mays cultivars Tietar and DKC 6575 |
|
