|
| Status |
Public on Jul 30, 2014 |
| Title |
WT differentiating SVZ cells-#3 |
| Sample type |
RNA |
| |
|
| Source name |
Subventricular zone cells
|
| Organism |
Mus musculus |
| Characteristics |
growth media: Differentiation media for 30 hr
age: post natal day 9
genotype/variation: Jmjd3F/+
|
| Growth protocol |
SVZ NSCs were passaged 5 times in proliferation medium(DMEM/F12/N2 – Invitrogen, 5% FCS - Hyclone, 20 ng ml-1 EGF, 20 ng ml-1 bFGF, 35 ?g ml-1 bovine pituitary extract – Peprotech) before switching to differentiation media (same as proliferation media exc
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol (Life Technologies), purified using the RNeasy Plus Mini Kit (Qiagen) and quantified using the NanoDrop spectrophotometer. Samples were checked for quality using an Agilent TapeStation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated from total RNA using an Ambion Illumina RNA amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 29, 2014 |
| Last update date |
Jul 30, 2014 |
| Contact name |
John Liu |
| E-mail(s) |
john.liu@ucsf.edu
|
| Organization name |
UCSF
|
| Street address |
35 Medical Center Way
|
| City |
SAN FRANCISCO |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE59888 |
Epigenetic activation of neuronal gene expression by JMJD3 is required for postnatal and adult brain neurogenesis |
|
