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Sample GSM1468739 Query DataSets for GSM1468739
Status Public on Aug 09, 2014
Title GHRHR-mutant little B6 male rep1
Sample type RNA
 
Channel 1
Source name Little B6 male liver
Organism Mus musculus
Characteristics strain: C57BL/6
age: 10 weeks
tissue: whole liver
Sex: male
Treatment protocol Polytron 10 seconds and processed with an Qiagen Rneasy Mini kit
Growth protocol Ad lib feeding and drinking. Initiated with DEN at 12 doa. Harvest whole liver at 10wks.
Extracted molecule total RNA
Extraction protocol Total RNA was amplified and labeled according to manufactures instructions. Agilent Two-Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
Label Cy3
Label protocol Agilent Two-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
 
Channel 2
Source name pooled mixed-sex whole liver
Organism Mus musculus
Characteristics strain: C57BL/6
age: 10 weeks
tissue: whole liver
Sex: male and female mix
Treatment protocol Polytron 10 seconds and processed with an Qiagen Rneasy Mini kit
Growth protocol Ad lib feeding and drinking. Initiated with DEN at 12 doa. Harvest whole liver at 10wks.
Extracted molecule total RNA
Extraction protocol Total RNA was amplified and labeled according to manufactures instructions. Agilent Two-Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
Label Cy5
Label protocol Agilent Two-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
 
 
Hybridization protocol Agilent Technologies Scanner G2505B US22502567 5um 16bit
Scan protocol Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
Description pooled reference RNA
Data processing Agilent Feature extraction versions 9.1 and 9..5 for background subtraction - localbackground and normalization Linear LOWESS, Goodspots were designated as those features with both channels r and g where controltype=0, issaturated=0,isfeatnonunifol=0,isfeatpopnol=0,iswellabovebg=1 in 80% of arrays
 
Submission date Aug 08, 2014
Last update date Aug 09, 2014
Contact name Christopher Oberley
E-mail(s) oberleychris@gmail.com
Organization name University of Wisconsin-Madison
Department Oncology
Street address 3809 Hillcrest Dr.
City Madison
State/province Wisconsin
ZIP/Postal code 53705
Country USA
 
Platform ID GPL4134
Series (1)
GSE60253 Murine Liver Cells: Control B6 males vs. little (GH-deficient) and Tfm (AR-null) males

Data table header descriptions
ID_REF
VALUE normalized log10 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
1 1.89E-01
2 0.00E+00
3 0.00E+00
4 0.00E+00
5 0.00E+00
6 0.00E+00
7 0.00E+00
8 0.00E+00
9 0.00E+00
10 0.00E+00
11 0.00E+00
12 0.00E+00
13 -1.24E-02
14 -1.30E-01
15 -1.08E-01
16 -7.35E-02
18 0.00E+00
19 0.00E+00
20 0.00E+00
21 6.61E-02

Total number of rows: 45018

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1468739_Little-rep1.txt.gz 14.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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