|
Status |
Public on Aug 09, 2014 |
Title |
GHRHR-mutant little B6 male rep1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Little B6 male liver
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 10 weeks tissue: whole liver Sex: male
|
Treatment protocol |
Polytron 10 seconds and processed with an Qiagen Rneasy Mini kit
|
Growth protocol |
Ad lib feeding and drinking. Initiated with DEN at 12 doa. Harvest whole liver at 10wks.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was amplified and labeled according to manufactures instructions. Agilent Two-Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
|
Label |
Cy3
|
Label protocol |
Agilent Two-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
|
|
|
Channel 2 |
Source name |
pooled mixed-sex whole liver
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 10 weeks tissue: whole liver Sex: male and female mix
|
Treatment protocol |
Polytron 10 seconds and processed with an Qiagen Rneasy Mini kit
|
Growth protocol |
Ad lib feeding and drinking. Initiated with DEN at 12 doa. Harvest whole liver at 10wks.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was amplified and labeled according to manufactures instructions. Agilent Two-Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
|
Label |
Cy5
|
Label protocol |
Agilent Two-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
|
|
|
|
Hybridization protocol |
Agilent Technologies Scanner G2505B US22502567 5um 16bit
|
Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
Description |
pooled reference RNA
|
Data processing |
Agilent Feature extraction versions 9.1 and 9..5 for background subtraction - localbackground and normalization Linear LOWESS, Goodspots were designated as those features with both channels r and g where controltype=0, issaturated=0,isfeatnonunifol=0,isfeatpopnol=0,iswellabovebg=1 in 80% of arrays
|
|
|
Submission date |
Aug 08, 2014 |
Last update date |
Aug 09, 2014 |
Contact name |
Christopher Oberley |
E-mail(s) |
oberleychris@gmail.com
|
Organization name |
University of Wisconsin-Madison
|
Department |
Oncology
|
Street address |
3809 Hillcrest Dr.
|
City |
Madison |
State/province |
Wisconsin |
ZIP/Postal code |
53705 |
Country |
USA |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE60253 |
Murine Liver Cells: Control B6 males vs. little (GH-deficient) and Tfm (AR-null) males |
|