labeled cRNA were prepared from 1~5 ug total RNA using the Agilent’s Quick Amp Labeling Kit
Hybridization protocol
Following fragmentation, 1.65 ug of cRNA were hybridized to the Agilent expression microarray according to the protocols provided by the manufacturer
Scan protocol
Arrays were scanned using the Agilent Technologies G2600D SG12494263
Data processing
Array data export processing and analysis was performed using Agilent Feature Extraction v11.0.1.1. Quantile normalization of log2(probes) values using library(preprocessCore) in R 3.0.2 (www.r-project.org)
