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Sample GSM147168 Query DataSets for GSM147168
Status Public on Jan 30, 2007
Title MyD88_ZYM_A
Sample type RNA
 
Source name Bone marrow-derived macrophages, MyD88 -/-, Zymosan stimulated, 120 minutes
Organism Mus musculus
Characteristics Bone marrow-derived macrophages from mice deficient in signaling adaptor molecule MyD88
Treatment protocol Macrophages were stimulated for 2 hours with 100 ug/ml zymosan
Growth protocol Macrophages were prepared from bone marrow by growth for 6 days in the presence of recombinant human M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000G.
Description MyD88 deficient bone marrow-derived macrophages with and without Zymosan stimulation
Data processing Raw CEL intensity data were RMA normalized using R/Bioconductor
 
Submission date Nov 27, 2006
Last update date Aug 02, 2019
Contact name Bruz Marzolf
E-mail(s) bmarzolf@systemsbiology.org
Phone 206-732-1352
Organization name Institute for Systems Biology
Street address
City Seattle
State/province WA
ZIP/Postal code 98103
Country USA
 
Platform ID GPL1261
Series (1)
GSE6376 Zymosan stimulation of MyD88-deficient bone marrow-derived macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE normalized log2 signal

Data table
ID_REF VALUE
1415670_at 8.7947379930079
1415671_at 11.1248406508653
1415672_at 10.5659387016965
1415673_at 7.45451198346664
1415674_a_at 9.00453474178313
1415675_at 8.59419054390956
1415676_a_at 11.1725213364545
1415677_at 8.5881194019972
1415678_at 9.5830531111395
1415679_at 10.1579412816160
1415680_at 9.17820823765694
1415681_at 9.45179755783974
1415682_at 7.15110841098685
1415683_at 10.6461978994030
1415684_at 8.30218976499622
1415685_at 8.46536569776022
1415686_at 10.0377422381127
1415687_a_at 12.9591761254478
1415688_at 8.96429031247609
1415689_s_at 6.17283587607872

Total number of rows: 45101

Table truncated, full table size 1246 Kbytes.




Supplementary file Size Download File type/resource
GSM147168.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM147168.EXP.gz 496 b (ftp)(http) EXP

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