|
Status |
Public on Jan 30, 2007 |
Title |
MyD88_ZYM_A |
Sample type |
RNA |
|
|
Source name |
Bone marrow-derived macrophages, MyD88 -/-, Zymosan stimulated, 120 minutes
|
Organism |
Mus musculus |
Characteristics |
Bone marrow-derived macrophages from mice deficient in signaling adaptor molecule MyD88
|
Treatment protocol |
Macrophages were stimulated for 2 hours with 100 ug/ml zymosan
|
Growth protocol |
Macrophages were prepared from bone marrow by growth for 6 days in the presence of recombinant human M-CSF
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000G.
|
Description |
MyD88 deficient bone marrow-derived macrophages with and without Zymosan stimulation
|
Data processing |
Raw CEL intensity data were RMA normalized using R/Bioconductor
|
|
|
Submission date |
Nov 27, 2006 |
Last update date |
Aug 02, 2019 |
Contact name |
Bruz Marzolf |
E-mail(s) |
bmarzolf@systemsbiology.org
|
Phone |
206-732-1352
|
Organization name |
Institute for Systems Biology
|
Street address |
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98103 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE6376 |
Zymosan stimulation of MyD88-deficient bone marrow-derived macrophages |
|
Relations |
Reanalyzed by |
GSE119085 |
Reanalyzed by |
GSE135324 |