|
| Status |
Public on Feb 02, 2007 |
| Title |
Ecoli_MG1655_5h_aerobic1 |
| Sample type |
RNA |
| |
|
| Source name |
Ecoli MG1655 aerobic LB 5h
|
| Organism |
Escherichia coli |
| Characteristics |
Genotype: MG1655
|
| Treatment protocol |
RNA isolation using RNAprotect and RNeasy kit (Qiagen),
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA (Rneasy kit; Qiagen) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA isolated using RNeasy kit (Qiagen), reverse transcribed and labeled using BioArray Terminal Labeling Kit (Enzo).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2-5 microg of cDNA were hybridized for 16 hr at 45C on GeneChip Antisense Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from E. coli MG1655 grown aerobically (5h) in Luria-Bertani medium.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) after electronic masking; scaling = 500
|
| |
|
| Submission date |
Dec 04, 2006 |
| Last update date |
Dec 05, 2006 |
| Contact name |
Christopher Swanson Reigstad |
| E-mail(s) |
reigstad@wustl.edu
|
| Phone |
314-518-5021
|
| Fax |
314-362-2156
|
| Organization name |
Washington University in St. Louis
|
| Department |
Center for Genome Sciences
|
| Lab |
Jeffrey I. Gordon
|
| Street address |
4444 Forest Park Blvd.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL199 |
| Series (1) |
| GSE6425 |
Expression data from time courses of E. coli MG1655 and UTI89 in vitro |
|
