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Status |
Public on Feb 02, 2007 |
Title |
Ecoli_UTI89_3h30m_anaerobic2 |
Sample type |
RNA |
|
|
Source name |
Ecoli UTI89 anaerobic LB 3h30m
|
Organism |
Escherichia coli |
Characteristics |
Genotype: UTI89
|
Treatment protocol |
RNA isolation using RNAprotect and RNeasy kit (Qiagen),
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA (Rneasy kit; Qiagen) was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
RNA isolated using RNeasy kit (Qiagen), reverse transcribed and labeled using BioArray Terminal Labeling Kit (Enzo).
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|
|
Hybridization protocol |
Following fragmentation, 2-5 microg of cDNA were hybridized for 16 hr at 45C on GeneChip Antisense Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Description |
Gene expression data from E. coli UTI89 grown anaerobically (3h30m) in Luria-Bertani medium.
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) after electronic masking; scaling = 500
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|
|
Submission date |
Dec 04, 2006 |
Last update date |
Dec 05, 2006 |
Contact name |
Christopher Swanson Reigstad |
E-mail(s) |
reigstad@wustl.edu
|
Phone |
314-518-5021
|
Fax |
314-362-2156
|
Organization name |
Washington University in St. Louis
|
Department |
Center for Genome Sciences
|
Lab |
Jeffrey I. Gordon
|
Street address |
4444 Forest Park Blvd.
|
City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63108 |
Country |
USA |
|
|
Platform ID |
GPL199 |
Series (1) |
GSE6425 |
Expression data from time courses of E. coli MG1655 and UTI89 in vitro |
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