|
| Status |
Public on Dec 01, 2014 |
| Title |
DC Donor 6 day 7 [30 Hy5TM] |
| Sample type |
RNA |
| |
|
| Source name |
DC Donor 6 day 7
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: DC
donor: 6
time point: day7
|
| Treatment protocol |
Cells were left untreated.
|
| Growth protocol |
CD14+ monocytes were cultured at 3×106 cells/ml in either (a) RPMI 1640 medium supplemented with 10% FBS, 2 mmol/L L-glutamine, penicillin (100 U/ml), streptomycin (100 μg/ml), 1000U/ml IL-4, 500U/ml GM-CSF; or (b) DMEM medium supplemented with 10% FBS, 2 mmol/L L-glutamine, penicillin (100 U/ml), streptomycin (100 μg/ml), gentamicin (50ng/ml), 100ng/ml M-CSF. Media was replenished every 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the miRNeasy kit (Qiagen) following manufacturer’s protocol. The RNA was quantitated using the NanoDrop (Thermo Scientific) and integrity assessed using the 2100 Bioanalyzer.
|
| Label |
Hy5TM
|
| Label protocol |
250ng of total RNA was used as input for miRCURY LNATM microRNA Hi-Power Labeling Kit. Labelling was performed on an automated Tecan hybridization station.
|
| |
|
| Hybridization protocol |
Hybridisation was performed by Exiqon.
|
| Scan protocol |
Scanning was performed by Exiqon.
|
| Description |
The samples were labeled using the miRCURY LNATM microRNA Hi-Power Labeling Kit, Hy3TM/Hy5TM and hybridized on the miRCURY LNATM microRNA Array (7th Gen)
|
| Data processing |
Data normalization was performed using Quantile normalization by Exiqon.
Quantile normalized signal
Fold change (Normalized Reads)
Expression analysis (ANOVA for Time - dendritic cells)
Expression analysis (ANOVA for Time - macrophages)
Expression analysis for DC_1h vs. Monocyte
Expression analysis for MP_1h vs. Monocyte
|
| |
|
| Submission date |
Aug 27, 2014 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Jezrom Bokcaerin Self-Fordham |
| E-mail(s) |
jez.fordham@gmail.com
|
| Phone |
7735247565
|
| Organization name |
UIC
|
| Department |
Periodontics
|
| Lab |
461
|
| Street address |
801 S Paulina
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL19128 |
| Series (1) |
| GSE60839 |
miRCURY LNATM microRNA Array (7th Gen) profiling of monocyte-derived macrophages and monocyte-derived dendritic cells. |
|
