|
| Status |
Public on Aug 28, 2015 |
| Title |
Longissimus cervicisMuscle_<1hrPostMortem_rep3 (7021) |
| Sample type |
RNA |
| |
|
| Source name |
Muscle tissue collected < 1hr post-mortem before electrical-stimulation
|
| Organism |
Bos indicus x Bos taurus |
| Characteristics |
residual shear force (nes): HIGH
tissue: Longissimus cervicis
gender: Steer
age: aprx. 18month
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1. RNA was extracted from aprx. 100-200 mg muscle sample using TRI Reagent and BCP (Molecular Research Center)
2. RNA was tested for quality using Agilent 2100 Series Bioanalyzer
3. RNA with RIN > 8.0 were treated with DNase (Invitrogen) and column purified using RNeasy mini-kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled according to Agilent One-Color microarray-based gene expression analysis; low input Quick amp labeling protocol (v.6)
|
| |
|
| Hybridization protocol |
RNA was hybridized to Agilent bovine 4x44K slides according to the hybridization procedures in the QuickAmp Labeling kit manual. Hybridized slides were also washed and dried according to that protocol.
|
| Scan protocol |
Slides were scanned on Agilent Technologies Scanner G2505C US85003609.
|
| Description |
Bos taurus x Bos indicus F2
Biological replicate 3 of 47: high v low residual shear force
|
| Data processing |
Agilent GeneSpring software (v 11.0.02) embedded functions were used for data normalization (quantile) and intepretation. Normalized to baseline median expression across all arrays, minimum threshold 1.0.
|
| |
|
| Submission date |
Aug 29, 2014 |
| Last update date |
Aug 28, 2015 |
| Contact name |
Robert Neil Vaughn |
| E-mail(s) |
bobv527@gmail.com
|
| Phone |
9794502765
|
| Organization name |
Texas A&M University
|
| Department |
Soil and Crop Sciences
|
| Lab |
New Beasley Lab
|
| Street address |
0965 Agronomy
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77845 |
| Country |
USA |
| |
|
| Platform ID |
GPL11649 |
| Series (1) |
| GSE60935 |
Longissimus cervicis muscle of beef steers with divergent Warner Bratzler Shear Force (WBSF) residuals following different post-mortem treatments (electrically stimulated v. non-stimulated) |
|
